What does the anti-phospholipid antibody (aPL) test, specifically anti-phospholipid a2 receptor antibody, test for?

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From the Guidelines

The anti-phospholipid a2 receptor antibody test is used to detect antibodies against the phospholipase A2 receptor, which is a key marker for primary membranous nephropathy, not antiphospholipid syndrome. According to the latest guidance from the ISTH-SSC subcommittee on lupus anticoagulant/antiphospholipid antibodies 1, the diagnosis of antiphospholipid syndrome (APS) relies on the detection of lupus anticoagulant (LA), anti-cardiolipin antibodies (aCL), and anti-beta2 glycoprotein I antibodies (aβ2GPI). The anti-phospholipid a2 receptor antibody test is not mentioned as a diagnostic criterion for APS in this guidance, suggesting that it serves a different purpose. Key points to consider when interpreting the results of this test include:

  • The test is primarily used to diagnose and monitor primary membranous nephropathy (PMN), a kidney disorder
  • The test detects autoantibodies against the phospholipase A2 receptor (PLA2R), which are present in approximately 70-80% of patients with PMN
  • Positive results strongly support a diagnosis of primary membranous nephropathy, while negative results may indicate secondary causes requiring further investigation
  • The test is also valuable for monitoring disease activity and treatment response, as antibody levels often decrease with successful therapy before clinical improvement becomes apparent. It is essential to differentiate between the classification criteria for APS and the assessment of antiphospholipid antibodies in clinical care, as the latter is broader and aimed at optimizing patient management 1.

From the Research

Anti-Phospholipid Antibody (aPL) Test

The anti-phospholipid antibody (aPL) test is used to detect the presence of antibodies that are associated with antiphospholipid syndrome (APS) [ 2, 3 ].

What the Test Measures

  • The test measures the levels of different types of aPL, including lupus anticoagulant (LAC), anticardiolipin (aCL), and anti-beta2-glycoprotein I (aβ2GPI) antibodies [ 2 ].
  • The test can also measure non-criteria aPL, such as antibodies against the domain I of beta2-glycoprotein (aDI) and antiphosphatidylserine-prothrombin (aPS/PT) antibodies, although these are not currently included in the diagnostic criteria for APS [ 2 ].
  • The anti-phospholipid a2 receptor antibody is not specifically mentioned in the provided studies as a separate entity, but it is implied to be part of the broader category of aPL [ 3 ].

Clinical Relevance

  • The aPL test is crucial for evaluating the risk of complications associated with APS, such as thrombosis and pregnancy morbidity [ 3, 4 ].
  • The test is also used to fulfill certain classification criteria for APS, which are applied as diagnostic markers in medical practice [ 3 ].
  • The results of the aPL test can help identify patients at risk of APS and guide treatment decisions [ 2, 5 ].

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Testing for antiphospholipid antibodies: Advances and best practices.

International journal of laboratory hematology, 2020

Research

Antiphospholipid antibody testing.

Medicina clinica, 2024

Research

Antiphospholipid syndrome, antiphospholipid antibodies, and stroke.

International journal of stroke : official journal of the International Stroke Society, 2023

Research

Antiphospholipid Syndrome: A Comprehensive Clinical Review.

Journal of clinical medicine, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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