What is the half-life of Humalog (insulin lispro)?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: November 12, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Half-Life of Humalog (Insulin Lispro)

The elimination half-life of insulin lispro (Humalog) is approximately 44 minutes following intravenous administration, with a clearance of 32 L/hour. 1

Pharmacokinetic Profile

The FDA label for LYUMJEV (insulin lispro-aabc), which shares the same active moiety as Humalog, provides the most precise pharmacokinetic data: 1

  • Median half-life: 44 minutes (following 15 unit IV bolus in healthy subjects) 1
  • Clearance: 32 L/hour (geometric mean, CV 22%) 1
  • Volume of distribution: 34 L (geometric mean, CV 30%) 1

Clinical Pharmacodynamics

While the elimination half-life is under one hour, the clinical duration of action is 3-4 hours after subcutaneous injection, which is what matters for patient management. 2

Key time-action characteristics for subcutaneous administration: 2

  • Onset: 5 minutes 2
  • Peak effect: 1-2 hours 2
  • Duration: 3-4 hours 2

Absorption Characteristics

After subcutaneous injection, insulin lispro demonstrates: 1

  • Appearance in circulation: Approximately 1 minute after injection 1
  • Time to 50% maximum concentration: 13 minutes 1
  • Time to maximum concentration (Tmax): 57 minutes 1
  • Absolute bioavailability: Approximately 65% (abdomen, deltoid, thigh) 1

Clinical Implications

The rapid elimination half-life and short duration of action provide important clinical advantages: 3, 4

  • Better matching of insulin action to postprandial glucose excursions 3
  • Reduced risk of delayed hypoglycemia, particularly nocturnal episodes 3, 5
  • Ability to inject immediately before or even after meals (within 15 minutes) 3, 6

Important Caveats

Patients must understand that the rapid onset (5 minutes) combined with the short half-life means hypoglycemia can develop quickly if food intake is delayed or inadequate. 2, 7 This is a critical safety consideration distinguishing lispro from regular human insulin, which has a slower onset of 15 minutes and longer duration of 6-8 hours. 2

Day-to-day variability in pharmacokinetics is relatively low: 1

  • Total exposure (AUC): 10% coefficient of variation 1
  • Maximum concentration: 16% coefficient of variation 1

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Insulin lispro: a new quick-acting insulin analogue.

Expert opinion on investigational drugs, 1997

Research

[Humalog® 200 U/ml KwikPen™].

Revue medicale de Liege, 2015

Guideline

Insulin Lispro Dosing and Management for Diabetic Patients

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.