How do I prepare a 1:10,000 concentration of adrenaline (epinephrine) from a 1:1000 ampule for patients in cardiac arrest and anaphylaxis?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: November 17, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Preparing 1:10,000 Adrenaline for Cardiac Arrest vs. Anaphylaxis

Critical Distinction: DO NOT Prepare 1:10,000 for Anaphylaxis

For anaphylaxis, you should NEVER dilute your 1:1000 ampule to 1:10,000 concentration—use the 1:1000 concentration intramuscularly as supplied. 1, 2 The 1:10,000 dilution is exclusively for intravenous use in cardiac arrest, not for anaphylaxis treatment. 1, 3


Patient 1: Cardiac Arrest

Preparation of 1:10,000 Solution

  • Take 1 mL of 1:1000 adrenaline (1 mg) and dilute it with 9 mL of normal saline to create 10 mL of 1:10,000 solution (0.1 mg/mL). 1
  • Each 1 mL of this diluted solution now contains 0.1 mg (100 mcg) of adrenaline. 1

Dosing for Cardiac Arrest

  • Administer 1 mg (10 mL of 1:10,000 solution) intravenously every 3-5 minutes during ongoing cardiac arrest. 1, 4
  • For refractory cardiac arrest, consider escalating to 3-5 mg administered intravenously over 3 minutes, followed by 4-10 mg/min infusion if needed. 1
  • Prolonged resuscitation efforts are encouraged in anaphylaxis-induced cardiac arrest, as patients are often young with healthy cardiovascular systems. 1

Patient 2: Anaphylaxis (NOT in Cardiac Arrest)

DO NOT Dilute—Use 1:1000 Intramuscularly

  • Administer 0.5 mL of 1:1000 adrenaline (0.5 mg) intramuscularly into the vastus lateralis (anterolateral thigh) immediately. 1, 2
  • The intramuscular route achieves peak plasma concentrations in 8±2 minutes compared to 34±14 minutes with subcutaneous injection. 2
  • Repeat the same dose every 5 minutes if symptoms fail to resolve or worsen. 2
  • There is no maximum number of intramuscular doses—continue every 5 minutes until symptoms resolve, with most patients requiring 1-2 doses but some needing more. 2

When to Consider Intravenous Adrenaline in Anaphylaxis

  • Only consider intravenous adrenaline if the patient fails to respond to multiple intramuscular doses and has severe refractory hypotension or bronchospasm. 1, 5
  • If intravenous administration is necessary, use 50 mcg (0.5 mL of 1:10,000 solution) as an initial adult dose, titrated carefully to response. 1
  • Intravenous adrenaline carries significant risk of dilution/dosing errors and serious cardiac adverse effects—the intramuscular route is safer and preferred for first-line treatment. 2, 3

Critical Safety Points

Common Fatal Error to Avoid

  • Never administer 1:1000 concentration intravenously—this represents a 10-fold overdose and can cause severe cardiac complications including ventricular arrhythmias, myocardial dysfunction, and death. 3, 6
  • Confusion between 1:1000 (for IM use) and 1:10,000 (for IV use) is a well-documented cause of iatrogenic overdose. 3
  • Clearly label all syringes with concentration, route, and indication to prevent medication errors. 3

Concurrent Management for Anaphylaxis

  • Position the patient supine with legs elevated—never allow standing or walking, as this increases mortality risk. 2
  • Administer normal saline bolus of 1000-2000 mL for adults with hypotension. 2
  • Call for emergency assistance immediately, even if symptoms initially improve. 2
  • Consider adjunctive therapy with antihistamines (chlorphenamine 10 mg IV) and corticosteroids (hydrocortisone 200 mg IV) after adrenaline administration. 1

Monitoring Requirements

  • For intravenous adrenaline administration, monitor blood pressure and pulse every minute with continuous electrocardiographic monitoring if available. 1
  • Watch for signs of excessive vasoconstriction including cold extremities and decreased urine output. 7

Summary Algorithm

Cardiac Arrest Patient:

  1. Dilute 1 mL of 1:1000 adrenaline with 9 mL saline → 10 mL of 1:10,000
  2. Give 10 mL (1 mg) IV push every 3-5 minutes
  3. Continue until return of spontaneous circulation

Anaphylaxis Patient (NOT in arrest):

  1. DO NOT dilute—use 1:1000 as supplied
  2. Give 0.5 mL (0.5 mg) IM into thigh immediately
  3. Repeat same dose every 5 minutes if needed
  4. Only consider IV route if multiple IM doses fail (use 1:10,000 dilution, 50 mcg initial dose)

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Anaphylaxis Recognition and Treatment

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Adrenaline dosage during cardiopulmonary resuscitation: a critical review.

European journal of emergency medicine : official journal of the European Society for Emergency Medicine, 1994

Research

Cardiac Arrest Caused by Anaphylaxis Refractory to Prompt Management.

The American journal of emergency medicine, 2022

Guideline

Norepinephrine Drip Administration Protocol

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.