What is the recommended dose of cefazolin (Ancef) for Group B streptococcal (GBS) prophylaxis?

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Cefazolin Dosing for GBS Prophylaxis

For GBS prophylaxis in penicillin-allergic pregnant women without high-risk allergy features, administer cefazolin 2 g IV as the initial dose, followed by 1 g IV every 8 hours until delivery. 1

Dosing Regimen

  • Initial loading dose: 2 g IV 1, 2, 3
  • Maintenance dose: 1 g IV every 8 hours until delivery 1, 2, 3
  • The first dose should ideally be administered at least 2 hours before delivery to achieve maximum preventive effect, as effectiveness increases from 71% to 89% when given ≥2 hours before delivery 4

When Cefazolin is Indicated

  • Cefazolin is the preferred alternative for penicillin-allergic pregnant women who do NOT have a history of anaphylaxis, angioedema, respiratory distress, or urticaria following penicillin or cephalosporin administration 1
  • This recommendation is based on pharmacologic data showing cefazolin achieves effective intraamniotic concentrations 1
  • Research confirms that cefazolin concentrations exceed the MIC₉₀ for GBS in nearly all maternal, fetal, and amniotic fluid samples when using this dosing regimen 5

Critical Contraindications

Do NOT use cefazolin in patients with high-risk penicillin allergy features including: 1

  • History of anaphylaxis
  • History of angioedema
  • History of respiratory distress or urticaria following penicillin or cephalosporin administration
  • Approximately 10% of penicillin-allergic patients also have immediate hypersensitivity to cephalosporins 2

Alternative Agents for High-Risk Allergies

For patients at high risk for anaphylaxis: 1, 3

  • Clindamycin 900 mg IV every 8 hours (if GBS isolate is susceptible to both clindamycin and erythromycin)
  • Vancomycin 1 g IV every 12 hours (if susceptibility testing unavailable or isolate is resistant)

Pharmacokinetic Evidence Supporting This Dosing

  • Population pharmacokinetic modeling demonstrates that the standard 2 g loading dose followed by 1 g every 8 hours provides adequate maternal and umbilical cord exposure for up to 12 hours 6
  • At delivery, maternal and arterial umbilical cord unbound cefazolin concentrations exceeded the target of 1 mg/L in 95.8% and 91.7% of cases respectively 6
  • All GBS isolates remain universally susceptible to cefazolin, supporting its continued use 7

Common Pitfalls to Avoid

  • Never use cefazolin in patients with documented high-risk penicillin allergy - approximately 10% will cross-react 2
  • Do not underdose - the 2 g loading dose is essential to achieve rapid therapeutic levels 1
  • Do not extend the dosing interval beyond 8 hours - this is longer than typical cefazolin dosing due to pregnancy-related pharmacokinetic changes 1
  • Verify allergy history - many reported penicillin allergies are not true IgE-mediated reactions, and patients may be candidates for penicillin rather than cefazolin 2

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Treatment of Group B Streptococcal UTI in Pregnant Women

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Group B Streptococcus Treatment Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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