What is GHK-Cu (Glycine-Histidine-Lysine-Copper)?

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Last updated: November 20, 2025View editorial policy

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What is GHK-Cu?

GHK-Cu (glycyl-L-histidyl-L-lysine-copper) is a naturally occurring tripeptide-copper complex found in human plasma, saliva, and urine that declines with age, but it currently lacks established clinical guidelines, safety data, or FDA approval for therapeutic use. 1

Biochemical Properties

  • GHK is a tripeptide consisting of glycine, histidine, and lysine that has high affinity for copper(II) ions, forming the chelate complex GHK-Cu 2, 3
  • Plasma levels average approximately 200 ng/ml at age 20 but decline to around 80 ng/ml by age 60 4
  • The molecule exists both as copper-free GHK and as the copper complex GHK-Cu, with both forms showing biological activity 5

Proposed Biological Mechanisms (Research-Based)

  • GHK-Cu stimulates synthesis and breakdown of collagen, glycosaminoglycans (particularly dermatan sulfate and heparan sulfate), and the small proteoglycan decorin 2, 6
  • The compound modulates metalloproteinase activity and their inhibitors, affecting tissue remodeling 2
  • Research suggests it can up- and downregulate thousands of human genes, potentially affecting epigenetic pathways 2, 3
  • In vitro studies show antioxidant and anti-inflammatory properties 3, 4

Lack of Clinical Evidence and Guidelines

  • No major medical societies (European League Against Rheumatism, American College of Rheumatology) include GHK-Cu in their treatment guidelines 1
  • The safety profile, appropriate dosing, drug interactions, and long-term effects in humans remain unknown 1
  • Most evidence comes from in vitro studies, animal models, and cosmetic applications rather than rigorous clinical trials 2, 4

Current Applications (Non-Medical)

  • GHK-Cu is used in cosmetic products for skin care, where it has been reported to improve skin elasticity, reduce wrinkles, and increase keratinocyte proliferation 2, 5
  • Research shows wound healing effects in animal models (rats, mice, pigs, dogs) but human clinical data is limited 2

Critical Caveats

  • The compound is not FDA-approved for therapeutic injection or systemic use 1
  • Copper toxicity is a concern, particularly in patients with abnormal liver function or cholestasis, as copper is primarily excreted through bile 7
  • The relationship between copper-free GHK and copper-GHK effects requires further clarification 5
  • Proposed therapeutic uses for neurodegeneration, COPD, and cancer remain purely speculative without clinical trial data 2, 3

References

Guideline

GHK-Cu Injections: Lack of Clinical Guidelines and Established Use

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

The potential of GHK as an anti-aging peptide.

Aging pathobiology and therapeutics, 2020

Research

Stem cell recovering effect of copper-free GHK in skin.

Journal of peptide science : an official publication of the European Peptide Society, 2012

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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