What laboratory tests are indicated for follow-up of a pituitary (hypophyseal) mass?

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From the Guidelines

Laboratory Tests for Pituitary Mass Follow-up

Baseline laboratory tests for follow-up of a pituitary mass should include evaluation of ACTH, cortisol, TSH, free T4, and electrolytes.

  • Additional tests may be considered based on clinical presentation, such as:
    • LH and testosterone in males
    • FSH and estrogen in premenopausal females with symptoms like fatigue, loss of libido, mood changes, or oligomenorrhea
    • Standard-dose ACTH stimulation testing for indeterminate results (AM cortisol < 3 mg/dL and < 15 mg/dL) 1
  • MRI brain with pituitary or sellar cuts is recommended for all patients with new hormonal deficiencies, particularly those with multiple endocrine abnormalities, severe headaches, or complaints of vision changes 1
  • Thyroid function tests and regular MRI surveillance are suggested for disease monitoring in patients with confirmed TSHomas, although the optimal surveillance schedule is unknown and should be individualized 1
  • Pituitary hormone assessment should be undertaken as indicated clinically to confirm the absence of pituitary hormone excess from a functioning adenoma or the presence of hormone deficiency 1
  • Visual acuity and visual fields assessment is mandated in cases of suspected visual deterioration, which is a well-described presentation of non-functioning pituitary macroadenoma (NFPA) in children and young people (CYP) 1

From the Research

Laboratory Tests for Pituitary Mass Follow-up

The following laboratory tests are indicated for the follow-up of a pituitary mass:

  • Serum prolactin levels for small tumors 2
  • Laboratory evaluation for metabolic problems, such as thyroid and adrenal function 2, 3
  • Insulin-like growth factor 1 (IGF-1) and growth hormone levels for growth hormone-secreting tumors 4, 5
  • Late-night salivary cortisol level for adrenocorticotropic hormone (ACTH)-secreting tumors 4
  • Thyroid-stimulating hormone (TSH) levels for TSH-secreting tumors 4, 5
  • Gonadal, thyroid, and adrenal function tests for all patients with pituitary tumors 3

Additional Evaluations

Additional evaluations that may be necessary for the follow-up of a pituitary mass include:

  • Ophthalmological examination, including formal visual field testing, for patients with tumors compressing the optic chiasm 5, 2, 6
  • Imaging procedures, such as magnetic resonance imaging (MRI), to determine the presence, size, and extent of the lesion 3, 6
  • Stimulation and suppression tests for pituitary hormones in selected situations 3

Specific Tumor Types

For specific types of pituitary tumors, the following laboratory tests and evaluations may be indicated:

  • Prolactinomas: serum prolactin levels, dopamine agonist therapy 4, 5, 3
  • Growth hormone-secreting tumors: IGF-1 and growth hormone levels, somatostatin analogues, pegvisomant 4, 5, 3
  • ACTH-secreting tumors: late-night salivary cortisol level, petrosal sinus sampling for ACTH, adrenal steroidogenesis inhibitors 4, 3
  • TSH-secreting tumors: TSH levels, somatostatin analogues 4, 5

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Incidentally discovered pituitary masses: pituitary incidentalomas.

Expert review of endocrinology & metabolism, 2010

Research

Diagnosis and treatment of pituitary adenomas.

Minerva endocrinologica, 2004

Research

Benign and Malignant Tumors of the Pituitary Gland.

Advances in experimental medicine and biology, 2023

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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