What laboratory tests are indicated for follow-up of a pituitary (hypophyseal) mass?

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Last updated: February 12, 2025View editorial policy

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From the Guidelines

Pituitary mass follow-up laboratory tests should include systematic pituitary hormone assessment.

Indicated Tests

  • Thyroid function tests: TSH, free T4 1
  • Adrenal function tests: ACTH, cortisol or 1 mcg cosyntropin stimulation test 1
  • Gonadal hormones: testosterone in men, estradiol in women, FSH, LH 1
  • GH excess testing: for patients with excess height or acromegalic features, with or without delayed or arrested puberty or family history of pituitary adenoma 1

Additional Evaluations

  • MRI brain with pituitary or sellar cuts: for all patients with new hormonal deficiencies, multiple endocrine abnormalities, severe headaches, or complaints of vision changes 1
  • Genetic assessment: for all patients with a pituitary adenoma to inform management and family surveillance, particularly for those with GH and prolactin excess 1
  • Visual review: as part of a multidisciplinary assessment and care approach 1
  • Histopathology: as part of a multidisciplinary assessment and care approach 1

From the Research

Laboratory Tests for Pituitary Mass Follow-up

The following laboratory tests are indicated for the follow-up of a pituitary (hypophyseal) mass:

  • Serum prolactin levels for small tumors 2
  • Laboratory evaluation for metabolic problems if present 2
  • Assessment for hypopituitarism, clinically and biochemically, if the mass is large 2
  • Insulin-like growth factor 1 levels and growth hormone levels for growth hormone-secreting tumors 3
  • Late-night salivary cortisol level as a screening test for adrenocorticotropic hormone (ACTH)-secreting tumors 3
  • Petrosal sinus sampling for ACTH to distinguish a pituitary from an ectopic source 3
  • Thyroid-stimulating hormone levels for thyroid-stimulating hormone-secreting tumors 3, 4

Additional Evaluations

Additional evaluations that may be necessary for the follow-up of a pituitary mass include:

  • Detailed history and physical examination to look for signs of functional or 'subclinically' functional tumor 2
  • Enhanced MRI if the mass was discovered on a computed tomography scan 2
  • Ophthalmological examination, including formal visual field testing, if the mass is compressing the optic chiasm 5, 2, 6
  • Assessment of hypothalamic-pituitary hormonal function 6

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Incidentally discovered pituitary masses: pituitary incidentalomas.

Expert review of endocrinology & metabolism, 2010

Research

Pituitary tumors. Current concepts in diagnosis and management.

The Western journal of medicine, 1995

Research

Benign and Malignant Tumors of the Pituitary Gland.

Advances in experimental medicine and biology, 2023

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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