Daptomycin 500 mg Reconstitution Volume
Reconstitute 500 mg daptomycin with 10 mL of 0.9% sodium chloride injection to achieve a final concentration of 50 mg/mL. 1
Reconstitution Procedure
The FDA-approved reconstitution process requires specific technique to prevent foaming and ensure complete dissolution 1:
Remove the polypropylene flip-off cap and wipe the rubber stopper with an alcohol swab, allowing it to dry before proceeding 1
Slowly transfer exactly 10 mL of 0.9% sodium chloride injection through the center of the rubber stopper into the vial, pointing the transfer needle toward the wall of the vial (not directly onto the powder) 1
Use a beveled sterile transfer needle that is 21 gauge or smaller in diameter, or a needleless device for the transfer 1
Ensure all powder is wetted by gently rotating (not shaking) the vial, then allow it to stand undisturbed for 10 minutes 1
Gently rotate or swirl the vial contents for a few minutes as needed to obtain a completely reconstituted solution 1
Critical Reconstitution Warnings
AVOID vigorous agitation or shaking during or after reconstitution to minimize foaming, which can interfere with proper dissolution 1
Only use 0.9% sodium chloride injection for reconstitution—daptomycin is NOT compatible with dextrose-containing diluents 1
Do not use ReadyMED® elastomeric infusion pumps as an impurity (2-mercaptobenzothiazole) leaches from this pump system into daptomycin solution 1
Stability After Reconstitution
Reconstituted solution is stable for 12 hours at room temperature or up to 48 hours if refrigerated at 2°C to 8°C (36°F to 46°F) 1
If further diluted for infusion, the combined storage time (reconstituted solution in vial plus diluted solution in infusion bag) must not exceed 12 hours at room temperature or 48 hours under refrigeration 1
No preservative or bacteriostatic agent is present—aseptic technique is mandatory and unused portions must be discarded 1