What is cortisol?

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Cortisol: A Glucocorticoid Hormone

Cortisol is a glucocorticoid hormone, the dominant naturally occurring glucocorticoid in humans, produced in the zona fasciculata of the adrenal cortex. 1

Classification and Chemical Nature

  • Cortisol is classified as a steroid hormone that functions as both a glucocorticoid (its primary action) and possesses mild mineralocorticoid (salt-retaining) properties 2

  • It is a lipophilic molecule (pregn-4-ene-3,20-dione,11,17,21-trihydroxy) with molecular weight 362.46, which makes it highly protein-bound in plasma 1, 2

Physiological Characteristics

Protein Binding and Bioavailability

  • 60-80% of cortisol binds to cortisol-binding globulin (CBG), 15-35% binds to albumin, and only approximately 5-10% circulates as free, biologically active hormone 1, 3

  • The free fraction represents the biologically active component that exerts metabolic effects 3

Production and Regulation

  • Cortisol production is controlled by the hypothalamic-pituitary-adrenal (HPA) axis: corticotropin-releasing hormone (CRH) from the hypothalamus stimulates adrenocorticotropic hormone (ACTH) from the anterior pituitary, which then stimulates cortisol release 1

  • Approximately 20 mg of cortisol is released daily under normal conditions, with production increasing up to five-fold (100 mg/day) during major physiological stress such as surgery 1

  • Cortisol secretion follows a circadian rhythm with pulsatile release: reference ranges are 140-700 nmol/L at 0900 hours but only 80-350 nmol/L at midnight 1

Primary Functions

Metabolic Effects

  • Cortisol causes profound and varied metabolic effects including regulation of carbohydrate, lipid, and protein metabolism 2

  • It acts as a catabolic hormone, promoting protein breakdown and gluconeogenesis while affecting energy expenditure 4

Stress Response

  • Cortisol is released in response to stress and is essential for maintaining health and wellbeing, leading to significant morbidity when deficient or present in excess 3, 5

  • During stress responses (fight-or-flight), adrenal release of epinephrine and cortisol allows threats to be addressed through physiological changes including increased heart rate and blood pressure 1

Immune Modulation

  • Cortisol modifies the body's immune responses to diverse stimuli and possesses potent anti-inflammatory effects 2

Clinical Significance

  • Cortisol measurement is essential for diagnosing adrenal insufficiency (Addison's disease) and cortisol excess (Cushing's syndrome) 1, 6

  • Chronic exposure to excessive cortisol is associated with insulin resistance, visceral obesity, and metabolic syndrome 5

  • In critically ill patients, cortisol levels below 250-276 nmol/L suggest relative adrenal insufficiency, though interpretation must account for reduced binding proteins in severe illness 1

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Cortisol in metabolic syndrome.

Advances in clinical chemistry, 2024

Research

Determination of cortisol in serum, saliva and urine.

Best practice & research. Clinical endocrinology & metabolism, 2013

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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