What is the stability and shelf life of dexamethasone (corticosteroid) mixed with bacteriostatic water (preservative-containing water) for Intramuscular (IM) injection?

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Last updated: November 27, 2025View editorial policy

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Stability of Dexamethasone Mixed with Bacteriostatic Water for IM Injection

Dexamethasone sodium phosphate diluted with bacteriostatic saline (0.9% NaCl) remains stable for 28 days when stored at either room temperature (22°C) or refrigerated (4°C). 1

Storage Duration and Conditions

  • 28-day stability has been demonstrated for dexamethasone sodium phosphate injection (4 mg/mL) diluted to 1 mg/mL with bacteriostatic 0.9% sodium chloride 1
  • Samples retained at least 97.7% of original concentration throughout the 28-day period at both storage temperatures 1
  • No visual changes (discoloration or precipitation) occurred during this timeframe 1

Storage Temperature Options

  • Room temperature (22°C/72°F): Stable for 28 days 1
  • Refrigeration (4°C/39°F): Stable for 28 days 1
  • Both storage conditions showed equivalent stability with no clinically significant difference 1

Container Considerations

When stored in syringes (if applicable for your preparation):

  • Glass syringes: Stable for up to 91 days with >95% concentration retained 2
  • Plastic syringes: Stable for 35-55 days (depending on syringe size) with >93% concentration retained 2
  • Important caveat: After 18 months of storage in syringes, rubber plunger leachates can contaminate the solution, though this is well beyond practical clinical use 2

Critical Stability Factors

Oxidation susceptibility: Dexamethasone sodium phosphate is chemically prone to degradation by oxidation under certain conditions 3, 4

  • Some commercially available dexamethasone sodium phosphate products have shown oxidative degradation issues when stored long-term in original vials 4
  • The diluted preparation in bacteriostatic saline appears more stable than some commercial formulations 1

Practical Recommendations

  • Label with beyond-use date: 28 days from preparation date 1
  • Storage location: Either refrigerated or at controlled room temperature is acceptable 1
  • Inspect before use: Check for clarity, color changes, or particulate matter before each withdrawal 1
  • Avoid extended storage: Do not exceed 28 days even if solution appears clear 1

Common Pitfalls to Avoid

  • Do not assume longer stability: While some studies show stability beyond 28 days in certain containers, the conservative 28-day limit is evidence-based for bacteriostatic saline dilutions 1
  • Do not use if discolored: Any visual change indicates potential degradation 1
  • Avoid extreme storage: Keep away from direct light and temperature extremes outside the 4-22°C range 1

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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