Reconstitution Calculation for Retatrutide
To achieve a 0.5ml dose from 12mg of Retatrutide, you need to add 12ml of bacteriostatic water, which creates a concentration of 1mg/ml.
Calculation Method
The reconstitution volume is determined by working backward from your desired dose volume and the total drug amount available:
- Total drug amount: 12mg
- Desired dose volume: 0.5ml
- Required concentration: You need to determine what concentration allows 0.5ml to contain your target dose
The standard approach is to create a concentration that makes dosing practical. If you reconstitute 12mg with 12ml of bacteriostatic water, you create a 1mg/ml solution. This means:
- Each 0.5ml contains 0.5mg
- Each 1ml contains 1mg
- The full 12ml contains 12mg
Practical Considerations
You must first determine your target dose in milligrams before finalizing the reconstitution volume. The question asks about achieving "a 0.5ml dose" but doesn't specify the intended dose in milligrams. Common dosing scenarios based on clinical trial data include:
- For 0.5mg dose: Use 12ml bacteriostatic water (1mg/ml concentration), draw 0.5ml 1, 2
- For 1mg dose: Use 12ml bacteriostatic water (1mg/ml concentration), draw 1ml 1, 2
- For higher doses: Adjust concentration accordingly or draw larger volumes
Dosing Context from Clinical Trials
Retatrutide has been studied at various dose levels in clinical trials:
- Starting doses: Typically 1mg, 2mg, or 4mg weekly 1, 2
- Maintenance doses: Range from 4mg to 12mg weekly, with dose escalation protocols 1, 2
- The 12mg dose showed maximum efficacy with mean weight reduction of 24.2% at 48 weeks and HbA1c reduction of 2.02% at 24 weeks 1, 2
Storage and Stability
Once reconstituted, bacteriostatic water-containing solutions typically remain stable for 28 days when refrigerated, though specific stability data for retatrutide should be confirmed with the manufacturer's prescribing information. Standard practice for peptide medications suggests using reconstituted solutions within this timeframe 3.