Reconstitution of Retatrutide: Practical Calculation
To achieve a 1mg dose from a 12mg vial of Retatrutide, add 1.2mL of bacteriostatic water, which creates a concentration of 10mg/mL, allowing you to draw 0.1mL (10 units on an insulin syringe) for each 1mg dose.
Reconstitution Mathematics
The calculation follows basic dilution principles used in pharmaceutical preparation:
- Starting amount: 12mg of lyophilized Retatrutide powder
- Desired concentration: 10mg/mL (this creates easy-to-measure doses)
- Required diluent volume: 1.2mL of bacteriostatic water
- Resulting concentration: 12mg ÷ 1.2mL = 10mg/mL
Dosing After Reconstitution
With a 10mg/mL concentration:
- For 1mg dose: Draw 0.1mL (10 units on insulin syringe)
- Total doses per vial: 12 doses of 1mg each
- Volume per dose: 0.1mL provides precise, measurable aliquots
This concentration allows for accurate dosing using standard insulin syringes marked in units (where 10 units = 0.1mL).
Alternative Concentration Options
If you prefer different concentrations:
- 2.4mL bacteriostatic water = 5mg/mL concentration (draw 0.2mL for 1mg dose)
- 6mL bacteriostatic water = 2mg/mL concentration (draw 0.5mL for 1mg dose)
The 10mg/mL concentration (1.2mL diluent) is optimal because it minimizes injection volume while maintaining measurement precision with standard insulin syringes.
Storage and Stability Considerations
After reconstitution with bacteriostatic water:
- Refrigerate the reconstituted solution at 2-8°C (36-46°F)
- Protect from light during storage
- Use within 28 days of reconstitution (standard for peptide medications with bacteriostatic water)
- Inspect visually before each use; discard if particulates or discoloration appear
Important Clinical Context
While the provided evidence focuses on antimicrobial dilution techniques 1 and renal dosing adjustments 1, 2, these principles do not directly apply to Retatrutide reconstitution. Retatrutide is a triple hormone receptor agonist studied for obesity and type 2 diabetes 3, 4, 5, 6, 7, not an antimicrobial agent. The reconstitution approach described above follows standard pharmaceutical compounding principles for peptide medications administered subcutaneously.