How is a milk allergy diagnosed and treated?

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How to Determine Milk Allergy

Milk allergy diagnosis requires a detailed clinical history of reproducible symptoms following milk exposure, combined with either skin prick testing (SPT) or specific IgE (sIgE) testing to demonstrate sensitization, followed by confirmation with an oral food challenge when the diagnosis remains uncertain. 1

Diagnostic Approach

Step 1: Clinical History Assessment

Document the following specific details:

  • Timing of symptoms: IgE-mediated reactions occur within minutes to 2 hours after milk ingestion 1
  • Type of symptoms:
    • IgE-mediated: hives, angioedema, wheezing, vomiting, anaphylaxis 1
    • Non-IgE-mediated: chronic diarrhea, blood-streaked stools, failure to thrive, enteropathy 1
    • Mixed: eosinophilic esophagitis, atopic dermatitis 1
  • Reproducibility: symptoms must occur consistently with milk exposure 1
  • Age of onset: typically presents in the first year of life 1

Critical distinction: Milk allergy is immune-mediated and differs from lactose intolerance, which causes bloating, flatulence, and diarrhea due to lactase deficiency without immune involvement 1

Step 2: Allergy Testing

For IgE-mediated milk allergy:

  • Skin prick testing (SPT) or serum-specific IgE (sIgE) should be performed 1
  • A positive test indicates sensitization but does NOT confirm clinical allergy 1
  • Negative tests have high negative predictive value and can help rule out IgE-mediated allergy 1
  • Different laboratory systems (ImmunoCAP, Turbo-MP, Immulite) provide different measurements; predictive values from one system cannot be applied to others 1

Important caveat: In Danish cohorts, 54% of confirmed milk allergy was IgE-mediated while 46% was non-IgE-mediated 1. For non-IgE-mediated reactions, SPT and sIgE are typically negative 1.

Atopy patch testing (APT) is NOT recommended for routine evaluation of milk allergy due to lack of standardization and variable sensitivity/specificity 1

Step 3: Elimination Diet

  • Remove all cow's milk protein from the diet 2, 3
  • Expected timeline for symptom resolution:
    • IgE-mediated: improvement within days
    • Non-IgE-mediated: 1-4 weeks depending on type 3
    • Allergic proctocolitis: typically 1-2 weeks 1

Step 4: Oral Food Challenge (Confirmation)

Gold standard: Double-blind, placebo-controlled food challenge (DBPCFC) 2, 4, 5, 6

In clinical practice:

  • Open challenges under medical supervision are commonly used except when symptoms are severe (e.g., anaphylaxis) 3, 5
  • Open challenges can overestimate milk allergy and should be used to reject rather than confirm diagnosis 4
  • For definitive diagnosis, DBPCFC is mandatory 4

Challenge protocol considerations:

  • Perform in a medically supervised setting with emergency equipment available 5
  • Contraindicated if recent severe anaphylaxis 3
  • For non-IgE-mediated allergic proctocolitis with classic history and symptom resolution on elimination, many experts believe oral challenge is not required 1

Special Diagnostic Considerations

Non-IgE-Mediated Presentations

Allergic proctocolitis:

  • Diagnosis based on: medical history, resolution with elimination, and recurrence following oral challenge 1
  • Presents as mucoid, blood-streaked stools in otherwise healthy infants 1
  • Colonoscopy/biopsy not generally necessary but shows mucosal edema with eosinophil infiltration if performed 1

Food protein-induced enteropathy:

  • Presents with chronic diarrhea, steatorrhea (80% of cases), weight loss, and growth failure 1
  • Diagnosis based on clinical symptoms, resolution with elimination, and recurrence following oral challenge 1

Treatment

Primary management: Strict elimination of cow's milk protein from the diet 2, 3, 4

Formula substitutes for infants:

  • Extensively hydrolyzed whey or casein formulas are first-line 2, 4, 5
  • Amino acid-based formulas rarely indicated 4
  • Soy-based formulas may be used in resource-limited settings 3

Emergency preparedness for IgE-mediated allergy:

  • Prescribe epinephrine autoinjector for severe reactions 1
  • Prescribe antihistamines for mild reactions 1

Natural History and Re-challenge

  • Most children develop tolerance by 2-3 years of age for non-IgE-mediated disease 1
  • Majority regain tolerance within first 5 years for IgE-mediated disease 1, 4
  • Re-challenge timing: Attempt reintroduction every 6 months after initial year of elimination 3
  • Use graded milk ladder approach for reintroduction at home or supervised in hospital depending on severity 5

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Cow's Milk Protein Allergy.

Indian journal of pediatrics, 2024

Research

Clinical practice. Diagnosis and treatment of cow's milk allergy.

European journal of pediatrics, 2009

Research

BSACI guideline for the diagnosis and management of cow's milk allergy.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 2014

Research

Milk allergy in adults and children.

Nursing standard (Royal College of Nursing (Great Britain) : 1987), 2015

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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