What is the composition of Normal Saline (NS) 0.9%?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: December 8, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Composition of Normal Saline 0.9%

Normal Saline (NS) 0.9% is composed of 154 mmol/L of sodium (Na+) and 154 mmol/L of chloride (Cl-), with an osmolarity of 308 mOsm/L and a pH range of 5-6.5. 1

Detailed Electrolyte Composition

  • Sodium concentration: 154 mmol/L 1
  • Chloride concentration: 154 mmol/L 1
  • No other electrolytes: Unlike balanced crystalloids, 0.9% NaCl contains no potassium, calcium, magnesium, bicarbonate, or buffer substances 1
  • Osmolarity: 308 mOsm/L, making it isotonic (within the 280-310 mOsm/L range) 1
  • pH: 5-6.5, which is acidic compared to physiologic pH of 7.4 1

Comparison to Plasma

When compared to normal plasma composition, 0.9% saline is notably different:

  • Sodium: NS contains 154 mmol/L versus plasma's 142 mmol/L (approximately 8% higher) 1
  • Chloride: NS contains 154 mmol/L versus plasma's 103 mmol/L (approximately 50% higher) 1
  • Lacks physiologic electrolytes: Plasma contains potassium (4 mmol/L), calcium (2.4 mmol/L), magnesium (1 mmol/L), and bicarbonate (27 mmol/L), none of which are present in NS 1

Clinical Implications of Composition

The supraphysiologic chloride content has important clinical consequences:

  • Hyperchloremic metabolic acidosis: Large volumes of 0.9% saline can cause this condition due to the excessive chloride load 1
  • Renal effects: The high chloride concentration may cause renal vasoconstriction and increase the risk of acute kidney injury 1
  • Not truly "normal" or "physiological": Despite its name, the composition bears little resemblance to plasma and has minimal scientific basis for being called "normal" 2

Alternative Formulations

For context, balanced crystalloids have compositions closer to plasma:

  • Lactated Ringer's: Contains Na+ 130 mmol/L, Cl- 108 mmol/L, K+ 4 mmol/L, Ca2+ 0.9 mmol/L, Mg2+ 3 mmol/L, and lactate 27.6 mmol/L 1
  • Plasma-Lyte: Contains Na+ 140 mmol/L, Cl- 98 mmol/L, K+ 5 mmol/L, Mg2+ 1 mmol/L, acetate 27 mmol/L 1

Important Caveats

  • Sodium overload risk: When used to dilute drugs or keep catheters open, 0.9% saline is a major source of iatrogenic hypernatremia in intensive care settings 3
  • Historical misconception: The widespread use of 0.9% saline appears to be based on historical fallacy rather than physiologic rationale 2
  • Hemolysis concerns: Normal saline causes substantially more in vitro hemolysis than balanced solutions during red cell washing and storage 4

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

The history of 0.9% saline.

Clinical nutrition (Edinburgh, Scotland), 2008

Research

0.9% NaCl (Normal Saline) - Perhaps not so normal after all?

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis, 2018

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.