What are the typical pressure settings and application protocols for Negative Pressure Wound Therapy (NPWT) used on skin grafts?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: December 8, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

NPWT for Skin Grafts: Pressure Settings and Application Protocols

For skin grafts, use continuous negative pressure at -75 mmHg rather than the traditional -125 mmHg setting, maintain therapy for 4-7 days before first inspection, and recognize that lower pressures reduce inflammation while improving graft outcomes compared to standard wound NPWT protocols. 1, 2

Pressure Settings

Recommended Pressure Range

  • -75 mmHg is superior to -125 mmHg for split-thickness skin grafts based on molecular evidence showing reduced proinflammatory gene expression (S100A8 and Tenascin C downregulated 8-9 fold) and increased fibrinogen production at the lower pressure setting 1

  • The traditional -125 mmHg setting used for open wounds causes significantly more inflammation and less fibrinogen deposition in grafts within the first 3 hours of application 1

  • Lower pressure (-80 mmHg with gauze) produces thinner scar tissue (7 mm average depth) compared to higher pressure (-125 mmHg with foam, 18 mm average depth), resulting in more pliable reconstructed tissue 3

Continuous vs Intermittent

  • Continuous therapy is standard for skin grafts across all reported protocols 2, 1, 4

  • Intermittent therapy is not recommended for graft applications, as continuous pressure maintains consistent graft-to-bed contact during the critical imbibition and inosculation phases

Duration Before First Inspection

  • 4-7 days is the typical duration before first dressing change and graft inspection 2, 4

  • Specifically, 4 days of NPWT post-graft application was successful in achieving complete healing in complex wounds 2

  • Average duration of 5.6-7.0 days post-graft is reported in contemporary practice, with the shorter duration (5.6 days) associated with single-use disposable systems 4

  • This extended period without inspection differs markedly from open wound NPWT, where dressing changes typically occur every 48-72 hours

Practical Differences from Open Wound NPWT

Pressure Modifications

  • Reduce pressure by approximately 40-50% compared to open wounds (from -125 mmHg standard to -75 to -80 mmHg for grafts) 2, 1, 3

  • The lower pressure prevents mechanical disruption of the delicate graft-bed interface during early healing phases 1

Dressing Change Frequency

  • Extended dressing intervals (4-7 days) versus 2-3 days for open wounds to avoid disturbing graft adherence 2, 4

  • No intermediate inspections should occur during the initial imbibition period (first 48-72 hours) when graft survival is most vulnerable

Interface Material Considerations

  • Low-adherence dressings are preferred over standard foam to minimize trauma during eventual removal 2

  • Gauze-based NPWT at -80 mmHg produces less sclerotic scar tissue and better tissue pliability compared to foam-based systems at -125 mmHg 3

Pain Management

  • Pain levels are generally moderate (4-5 on visual analog scale) and tend to decrease as therapy progresses, with lower pressures causing less discomfort than the -125 mmHg standard 2

  • The reduced pressure setting (-75 mmHg) improves patient comfort without compromising graft outcomes 1

Clinical Pathway Differences

  • NPWT over grafts facilitates immediate outpatient discharge (0.0 days average hospital stay) compared to 6.0 days with traditional rental NPWT systems, when using disposable single-patient-use devices 4

  • The sealed system protects the graft from external contamination and eliminates the need for traditional bolster dressings

Molecular Effects Specific to Grafts

  • NPWT on grafts increases expression of FGFR1, ET-1, and 22 keratin proteins, promoting cellular migration, proliferation, and angiogenesis 1

  • These effects occur within the first 3 hours of application, during the critical imbibition phase 1

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.