What does Mixtard 50 HM (human insulin) Penfill contain?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: December 10, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Mixtard 50 HM Penfill Composition

Mixtard 50 HM Penfill contains 50% soluble human insulin (regular insulin) and 50% isophane human insulin (NPH insulin) in a premixed formulation.

Active Ingredients

  • 50% Human Regular Insulin: This is the rapid-acting component that provides immediate glucose control after injection 1, 2
  • 50% Human NPH Insulin: This is the intermediate-acting component that provides sustained insulin coverage throughout the day 1, 2

The "HM" designation indicates this is human insulin (as opposed to animal-derived insulin), and the formulation is designed for use in pen injection devices (Penfill cartridges) 1.

Pharmacokinetic Profile

  • The 50/50 ratio provides higher initial insulin concentrations and a greater early glucose-lowering response compared to 70/30 mixtures, making it particularly useful when greater initial postprandial glucose control is required 2
  • The regular insulin component has onset of action within 30 minutes and should ideally be administered 30 minutes before meals for optimal effect 3
  • The NPH component provides intermediate-acting coverage with onset at approximately 1 hour, peak at 6-8 hours, and duration of approximately 12 hours 3

Clinical Considerations

  • This 50/50 formulation is typically administered twice daily (before breakfast and before the evening meal) 2, 4
  • The higher proportion of rapid-acting insulin (50% vs 30% in standard 70/30 mixtures) makes it more appropriate for patients with high carbohydrate intake or those requiring better postprandial glucose control 4
  • Studies demonstrate that 50/50 mixtures produce equivalent total bioavailability to 70/30 mixtures but with enhanced early glucose control 2

Important Safety Points

  • Requires consistent meal timing and content due to the NPH peak action profile to minimize hypoglycemia risk 3
  • May carry higher risk of nocturnal hypoglycemia compared to long-acting insulin analogs due to the NPH component 3
  • Should be inspected before each use to ensure the suspension appears uniformly cloudy after gentle mixing 1

Human insulin formulations like Mixtard 50 HM represent a cost-effective alternative to insulin analogs, with availability at approximately $25-35 per vial in some settings 3.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Cost-Effective Insulin Options for Humana Medicare Advantage Patients

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Use of 50/50 Premixed Insulin Analogs in Type 2 Diabetes: Systematic Review and Clinical Recommendations.

Diabetes therapy : research, treatment and education of diabetes and related disorders, 2017

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.