What is the best test to diagnose gout?

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Last updated: December 16, 2025View editorial policy

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Best Test to Diagnose Gout

Synovial fluid aspiration with polarized light microscopy to identify monosodium urate (MSU) crystals is the gold standard diagnostic test for gout, demonstrating exceptional specificity (100%) and high sensitivity (84%) with a likelihood ratio of 566.60. 1

When to Perform Synovial Fluid Analysis

You should perform joint aspiration when:

  • The diagnosis is clinically uncertain or ambiguous 1
  • There is any suspicion of septic arthritis (gout and sepsis can coexist in the same joint) 1
  • The patient has not had a prior crystal-proven diagnosis 1
  • An experienced clinician is available who can aspirate the joint without substantial patient discomfort 1
  • A polarizing microscope and trained operator are accessible 1, 2

You can rely on clinical judgment alone when:

  • The presentation is classic (podagra with appropriate risk factors like male sex, hyperuricemia, no overlying skin wound) 1
  • No significant probability of infection exists 1
  • Joint aspiration would cause substantial patient discomfort or is technically not feasible 1

Critical Technical Requirements

The test requires specific expertise and equipment that significantly impacts accuracy 1:

  • Polarizing microscope to identify needle-shaped crystals with strong negative birefringence 3, 4
  • Trained operator experienced in crystal identification (inter-observer variability exists) 1
  • Proper specimen handling to preserve crystal integrity 2

Essential Pitfall to Avoid

Always perform Gram stain and synovial fluid culture even when MSU crystals are identified if septic arthritis is in the differential diagnosis. 1, 4 In reported case series, 73-76% of patients with coexistent gout and septic arthritis had positive cultures, and some required amputation or died from untreated infection 1

When Synovial Fluid Analysis Cannot Be Performed

If joint aspiration is not feasible, validated clinical prediction algorithms can achieve sensitivities and specificities greater than 80% 1, 3:

  • The 2015 ACR/EULAR classification criteria (score ≥8 points) demonstrate 92% sensitivity and 89% specificity with imaging 3
  • Key high-value clinical features include presence of tophi (odds ratio 7.29) and resolution of pain within 2 weeks (odds ratio 3.58) 3

Alternative Imaging Modalities

Dual-Energy CT (DECT) is the most accurate non-invasive test with sensitivity 85-100% and specificity 83-92% 2, 3, but current evidence is insufficient for the American College of Physicians to recommend it for routine acute gout diagnosis 1

Ultrasonography shows variable performance (sensitivity 37-100%, specificity 68-97%) depending on the specific signs assessed 2, 3, with the "double contour" sign being most specific (pooled specificity 88%) 3

Intercritical Period Diagnosis

MSU crystals can be identified in asymptomatic joints during pain-free intervals, with approximately 70% positive yield in previously affected joints 1, allowing definitive diagnosis even between acute attacks 1

What NOT to Rely On

Serum uric acid levels do not confirm or exclude gout 1:

  • Many people with hyperuricemia never develop gout 1
  • During acute attacks, serum uric acid may be normal 1
  • Serum uric acid alone has insufficient evidence for diagnostic utility 1

Plain radiographs are not useful for confirming early or acute gout 1, though they may show typical features in chronic disease and help with differential diagnosis 1

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Gout Diagnosis Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Diagnosis and Treatment of Gout

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Gout Diagnosis and Crystal Identification

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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