What are other helpful laboratory tests to determine the etiology of metabolic acidosis?

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From the Guidelines

To determine the etiology of metabolic acidosis, several laboratory tests beyond the basic metabolic panel are helpful, including urine organic acid analysis, which can aid in the diagnosis of organic acidemias, fatty acid oxidation disorders, and other metabolic disorders, as outlined in the 2018 update by the American College of Medical Genetics and Genomics 1. The anion gap calculation is essential as it divides metabolic acidosis into high anion gap and normal anion gap types.

  • Serum lactate levels should be measured to identify lactic acidosis, which can result from tissue hypoperfusion, sepsis, or certain medications.
  • Serum ketones help diagnose diabetic, alcoholic, or starvation ketoacidosis.
  • Toxicology screens can detect substances like salicylates, methanol, ethylene glycol, or propylene glycol that cause toxic acidosis.
  • Urine studies including pH, electrolytes, and urine anion gap assist in diagnosing renal tubular acidosis and evaluating renal acid excretion.
  • Plasma osmolal gap helps identify ingestion of alcohols or glycols. Additional helpful tests include:
  • Serum creatinine and BUN to assess kidney function
  • Liver function tests to evaluate hepatic causes
  • Medication review to identify drug-induced acidosis In specific cases, measuring:
  • D-lactate (for short bowel syndrome)
  • Pyruvate levels (for mitochondrial disorders)
  • Or performing genetic testing for inherited metabolic disorders may be warranted, as certain disorders such as organic acidemias can be identified through urine organic acid analysis 1. These tests collectively provide a comprehensive evaluation to pinpoint the specific cause of metabolic acidosis, which is crucial for appropriate management.

From the Research

Helpful Labs to Determine Etiology of Metabolic Acidosis

To determine the etiology of metabolic acidosis, several labs can be helpful, including:

  • Electrolyte panel to evaluate sodium, potassium, chloride, and bicarbonate levels 2
  • Arterial blood gas (ABG) to assess pH, PaCO2, and PaO2 2
  • Serum anion gap calculation to classify the disorder into normal (hyperchloremic) or elevated anion gap 2, 3
  • Blood urea nitrogen (BUN) and creatinine to assess renal function 4, 5
  • Glucose and ketone levels to evaluate for diabetic ketoacidosis 4, 5
  • A1C to assess glucose control 4, 5
  • Urinalysis to evaluate for ketones, glucose, and protein 4, 5
  • Complete blood count (CBC) with differential to evaluate for infection or other conditions 4, 5
  • Electrocardiography (ECG) to evaluate for cardiac complications 4, 5
  • Amylase, lipase, hepatic transaminase levels, troponin, creatine kinase, blood and urine cultures, and chest radiography to evaluate for other conditions that may be contributing to metabolic acidosis 5

Specific Conditions

For specific conditions, additional labs may be helpful, such as:

  • Diabetic ketoacidosis: glucose, ketone levels, electrolyte panel, BUN, creatinine, A1C, urinalysis, CBC, and ECG 4, 5
  • Chronic kidney disease: electrolyte panel, BUN, creatinine, serum anion gap, and venous pH 6

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Differential diagnosis of metabolic acidosis.

Emergency medicine clinics of North America, 2005

Research

Diabetic ketoacidosis: evaluation and treatment.

American family physician, 2013

Research

Diabetic Ketoacidosis: Evaluation and Treatment.

American family physician, 2024

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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