What is the recommended workup for pancreatic cancer?

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Last updated: December 26, 2025View editorial policy

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Pancreatic Cancer Workup

Begin with a pancreatic protocol CT scan of the chest, abdomen, and pelvis combined with baseline CA 19-9 measurement, liver function tests, and family history assessment as the primary diagnostic approach. 1

Initial Imaging Strategy

  • Multiphasic contrast-enhanced CT (pancreatic protocol) is the first-line imaging modality, including late arterial and portal venous phases, with >90% positive predictive value for determining unresectability 1
  • Chest CT with contrast is mandatory to assess for metastatic disease 1
  • Abdominal ultrasound is useful for initial examination when patients present with abdominal pain or jaundice, though accuracy for diagnosing pancreatic tumors is only 50-70% 2, 3
  • If jaundice is present from an obstructive head tumor, obtain imaging BEFORE biliary drainage or stenting to avoid artifacts 1
  • Imaging should be performed within 4 weeks before starting treatment 1

Laboratory Workup

  • Obtain baseline CA 19-9 (if no cholestasis present) for prognostic information and treatment monitoring 1
    • CA 19-9 demonstrates 83% sensitivity and is the most clinically useful tumor marker 1, 4
    • Critical caveat: CA 19-9 is undetectable in Lewis antigen-negative patients (5-10% of population) and is elevated in cholestasis, making it unreliable for screening or initial diagnosis 1
    • Elevated CA 19-9 >500 IU/ml indicates worse prognosis and should prompt consideration of neoadjuvant therapy before surgery 1
  • Liver function tests are mandatory 1
  • Complete blood counts are required 1

Advanced Imaging When Needed

  • MRI with MRCP should be used when CT is inconclusive, contraindicated, or for evaluating cystic lesions 2, 1
  • Endoscopic ultrasound (EUS) should be considered to evaluate small lesions not visible on CT and can complement staging by providing information on vessel invasion and lymph node involvement 2, 1
  • ERCP is indicated only if clinical suspicion remains high despite negative CT imaging, or to relieve bile duct obstruction 2, 1
    • In the preoperative setting, ERCP and biliary stenting should only be performed if surgery cannot be done expeditiously 2

Tissue Diagnosis Strategy

The approach to biopsy depends on resectability status:

  • For resectable masses in surgical candidates, proceed directly to surgery without preoperative biopsy 1
  • For unresectable or metastatic disease, biopsy confirmation is mandatory before initiating systemic therapy 1
  • EUS-guided fine needle aspiration is the preferred biopsy method with highest accuracy and lowest risk of tumor seeding 1
  • Avoid percutaneous biopsy in surgical candidates due to risk of tumor seeding 2, 1
  • If metastatic lesions are present, biopsy these under ultrasound or CT guidance 2, 1

Staging Evaluation

  • Use the TNM staging system developed by the AJCC-UICC 2
  • Staging laparoscopy should be considered to exclude clinically occult intra-abdominal and lymph node metastases, particularly for left-sided large tumors, high CA 19-9 levels, or when neoadjuvant treatment is considered 2, 1
    • Laparoscopy may detect small peritoneal and liver metastases changing therapeutic strategy in <15% of patients 2

Molecular Testing

  • Obtain KRAS and BRCA testing for all patients 1
  • For metastatic disease with KRAS wild-type tumors, assess microsatellite instability (MSI) status, NTRK fusion status, and other rare actionable fusions 1
  • Patients with family history or high-risk features should undergo genetic counseling 1
  • BRCA1, BRCA2, or PALB2 mutations indicate potential platinum therapy sensitivity 1

Tests to Avoid

  • PET scan has no role in the diagnosis or routine staging of pancreatic cancer 2
  • Bone scan should not be ordered for routine staging, as only a few patients present with bone involvement at diagnosis 2, 1

Multidisciplinary Review

  • Consultation should involve expertise from diagnostic imaging, interventional endoscopy, medical oncology, radiation oncology, surgery, and pathology before finalizing treatment decisions 1

Clinical Presentation Clues

  • Look for painless jaundice in head of pancreas tumors, or persistent back pain with marked rapid weight loss in body/tail tumors 1
  • New-onset diabetes in adults without predisposing features occurs in up to 10% of patients as the first presentation 1

References

Guideline

Pancreatic Cancer Diagnosis and Management

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Diagnosis of pancreatic cancer.

HPB : the official journal of the International Hepato Pancreato Biliary Association, 2006

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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