What screening tests are recommended for a transgender man taking testosterone (hormone replacement therapy) with a history of normal Pap smear (Papanicolau test) and HPV (Human Papillomavirus) vaccination, normal A1c (glycated hemoglobin) and lipid profile?

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Recommended Screening for Transgender Man on Testosterone

This transgender man should undergo cervical cancer screening with HPV testing now, and repeat A1c and lipid panel should also be performed. 1, 2

Cervical Cancer Screening Priority

All persons with a cervix require cervical cancer screening regardless of gender identity or testosterone use. 1 The key considerations are:

  • The last Pap smear was 6 years ago, which exceeds recommended screening intervals of 3 years for cytology alone or 5 years for HPV testing with cytology 1
  • Despite HPV vaccination, screening remains necessary as vaccines do not cover all oncogenic HPV types 1
  • Transgender men on testosterone have high rates of cervical cytology interpretation errors due to testosterone-induced atrophic changes, transitional cell metaplasia, and small cell findings that can mimic abnormalities 3, 4
  • The clinical history of testosterone therapy must be provided to the cytopathologist to avoid overcalling abnormalities 3

Recommended approach: Perform HPV testing with cytology (co-testing) now, ensuring the laboratory is informed of the patient's transgender status and testosterone use 1, 3

Metabolic Monitoring for Testosterone Therapy

Testosterone replacement requires regular monitoring of metabolic parameters. 1, 5 The evidence supports:

  • A1c should be repeated as it has been 1 year since last testing, and annual monitoring is recommended for adults with risk factors 2
  • Lipid panel should be repeated as testosterone therapy affects lipid metabolism, particularly lowering HDL cholesterol toward male reference ranges 5
  • Testosterone-treated transgender men show significant shifts in HDL values compared to cisgender women, requiring ongoing cardiovascular risk assessment 5
  • Baseline monitoring at 1-2 months after initiation, then every 3-6 months for the first year, then yearly is the standard protocol 1

Additional Monitoring Considerations

While not explicitly asked about, testosterone monitoring should include 1:

  • Hematocrit/hemoglobin (polycythemia risk)
  • Blood pressure assessment
  • Liver function if clinically indicated
  • Digital rectal examination is not applicable for transgender men without prostates

What Is NOT Needed

Mammography is not indicated for this patient 1:

  • Mammography screening begins at age 40-45 years for average-risk individuals 1
  • No age is provided in this case, but the 5-year transition timeline suggests likely younger than screening age
  • Testosterone therapy and chest masculinization surgery (if performed) further reduce breast tissue and cancer risk

Psychology referral is not a routine screening recommendation 1:

  • Mental health support may be beneficial but is not part of standard preventive screening protocols
  • No indication of psychological distress is mentioned in the case

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Annual Primary Care Visit Recommendations

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Clinical history of female-to-male transgender patients is needed to avoid misinterpretation of cervical Papanicolaou tests.

Cytopathology : official journal of the British Society for Clinical Cytology, 2023

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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