Is Bactrim (trimethoprim/sulfamethoxazole) safe to use in patients with Glucose-6-phosphate dehydrogenase (G6PD) deficiency?

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Last updated: December 29, 2025View editorial policy

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Bactrim Use in G6PD Deficiency

Bactrim (trimethoprim-sulfamethoxazole) can be used safely in patients with G6PD deficiency, as it is not among the seven medications definitively contraindicated in this condition, and real-world evidence demonstrates safe use in thousands of G6PD-deficient patients. 1, 2

Evidence-Based Safety Profile

Medications Actually Contraindicated in G6PD Deficiency

Only seven medications are definitively contraindicated in G6PD deficiency according to major medical societies 1:

  • Dapsone
  • Methylthioninium chloride (methylene blue)
  • Primaquine
  • Rasburicase
  • Nitrofurantoin
  • Phenazopyridine
  • Tolonium chloride (toluidine blue) 3

Notably, trimethoprim-sulfamethoxazole (Bactrim) is not on this list. 1, 3

Real-World Clinical Evidence

The most compelling evidence comes from a large 2024 real-world study examining 31,962 G6PD-deficient patients 2:

  • Hundreds to thousands of G6PD-deficient patients were prescribed sulfamethoxazole/cotrimoxazole safely without major hemolysis requiring hospitalization 2
  • Only 71 cases (0.2%) of major hemolysis occurred in the entire cohort, with 71.8% caused by fava beans, 8.5% by infections, and only 4.2% possibly medication-related (none definitively linked to Bactrim) 2

Clinical Trial Data

A prospective double-blind study specifically evaluated high-dose TMP-SMZ (50 mg/kg/day of SMZ) in G6PD-deficient patients 4:

  • 20 G6PD-deficient patients received TMP-SMZ for serious Staphylococcus aureus infections 4
  • Zero cases of hemolysis occurred in the TMP-SMZ group 4
  • Serial monitoring of hemoglobin, haptoglobin, bilirubin, and reticulocyte counts showed no evidence of hemolysis 4

FDA Label Acknowledgment

The FDA label for sulfamethoxazole states: "In glucose-6-phosphate dehydrogenase deficient individuals, hemolysis may occur. This reaction is frequently dose-related." 5 However, this precautionary statement reflects theoretical concern rather than clinical reality, as demonstrated by the evidence above.

Clinical Algorithm for Use

When Bactrim is Indicated:

  1. Proceed with treatment in G6PD-deficient patients when clinically indicated 2, 4

  2. Monitor for hemolysis signs during therapy 1:

    • Jaundice
    • Dark urine
    • Fatigue
    • Pallor
  3. Consider baseline and follow-up labs if using high doses or prolonged therapy 4:

    • Hemoglobin
    • Reticulocyte count
    • Bilirubin
    • Haptoglobin

Variant-Specific Considerations

The severity of G6PD deficiency varies by genetic variant 1:

  • Mediterranean variant (Gdmed): More severe reactions, requires closer monitoring 1
  • African variant (GdA-): Milder, self-limited reactions 6

However, even in Mediterranean variants, Bactrim has been used safely in clinical practice 2.

Special Clinical Scenarios

Pneumocystis jirovecii Pneumonia (PJP)

Bactrim remains the first-line therapy for PJP even in G6PD-deficient patients 7:

  • A case report documented successful 21-day high-dose TMP-SMZ treatment in a G6PD-deficient HIV patient with PJP without hemolysis 7
  • Alternative regimens (atovaquone, inhaled pentamidine) have suboptimal cure rates and should be reserved for true contraindications 6, 7

High-Risk Populations

AIDS patients may have increased adverse reactions to Bactrim (rash, fever, leukopenia), but this is unrelated to G6PD status 5. The decision to use Bactrim should be based on the underlying condition requiring treatment, not G6PD deficiency alone.

Common Pitfalls to Avoid

  • Do not confuse historical precautionary listings with actual clinical risk: Many medications were wrongly cited as causing hemolysis because they were given during infection-related hemolytic episodes 3
  • Do not withhold life-saving therapy: When Bactrim is the best treatment option (e.g., PJP, serious bacterial infections), G6PD deficiency alone should not prevent its use 7, 4
  • Do not over-test: Routine intensive monitoring is unnecessary in most cases; clinical vigilance for hemolysis symptoms is sufficient 2

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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