What is the recommended Pre-Exposure Prophylaxis (PrEP) regimen for women to prevent Human Immunodeficiency Virus (HIV) infection?

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PrEP for Women: Recommended Regimen and Implementation

Daily oral tenofovir disoproxil fumarate/emtricitabine (TDF/FTC) 300mg/200mg taken once daily is the recommended PrEP regimen for all women at risk of HIV infection. 1, 2, 3

Critical Timing Consideration for Women

Women require at least 7 consecutive days of daily dosing before achieving maximum protection, unlike men who have sex with men who achieve protection within 24 hours. 3 This difference exists because tenofovir concentrates at 10-fold lower levels in vaginal tissue compared to rectal tissue, with faster drug clearance. 1, 3 Daily adherence is absolutely critical for women—there is no on-demand or event-driven dosing option for women. 1, 3

Who Should Receive PrEP

Offer PrEP to women with any of the following risk factors: 2, 3

  • HIV-positive sexual partner
  • Inconsistent condom use with partners of unknown HIV status
  • Recent bacterial sexually transmitted infection diagnosis
  • Sexual network with HIV incidence >2% per year
  • Multiple sexual partners
  • Injection drug use

Pre-Initiation Requirements

Before prescribing PrEP, obtain the following tests: 1, 2, 3

  • Combined HIV antibody and antigen testing (if acute HIV suspected based on symptoms, add HIV RNA testing)
  • Serum creatinine with estimated creatinine clearance calculation
  • Hepatitis B surface antigen (HBsAg)
  • Hepatitis C antibody
  • Nucleic acid amplification testing for gonorrhea and chlamydia (genital and extragenital sites)
  • Syphilis serology
  • Pregnancy test

Do not initiate PrEP if HIV infection cannot be excluded. 1

Monitoring Schedule During PrEP Use

At 1 Month

  • Combined HIV antibody/antigen test 1, 2
  • Adherence assessment and side effect evaluation 1

Every 3 Months (Quarterly)

  • Combined HIV antibody/antigen test 1, 2, 3
  • STI screening (gonorrhea, chlamydia, syphilis) 1, 3
  • Pregnancy testing 1, 3
  • Estimated creatinine clearance 1, 3

Every 6-12 Months

  • Hepatitis C antibody testing 1, 2
  • More frequent renal monitoring if baseline creatinine clearance <90 mL/min, diabetes, or hypertension 2, 3

Special Populations

Pregnancy and Breastfeeding

Continue TDF/FTC during pregnancy and breastfeeding—it is safe with no documented adverse fetal effects. 2, 3 This is the preferred regimen during pregnancy. 2

Hepatitis B Co-infection

For HBsAg-positive women, do not abruptly discontinue TDF/FTC as this can cause acute hepatitis B flares or hepatic decompensation. 2 Consider indefinite continuation or transition to hepatitis B treatment if stopping PrEP, with close ALT/AST monitoring after discontinuation. 2, 3

Renal Impairment

TDF/FTC is not recommended for women with creatinine clearance <60 mL/min. 1 Consider tenofovir alafenamide/emtricitabine (TAF/FTC) as an alternative, though this has less robust efficacy data in women. 1, 2

Efficacy and Adherence

PrEP efficacy exceeds 90% when adherence is maintained with detectable drug levels. 2, 3 However, efficacy drops dramatically to 44% with suboptimal adherence. 3 Enhanced adherence counseling is essential for all women initiating PrEP. 3, 4 Meta-analysis data show that with 75% adherence, oral PrEP achieves significant effectiveness in women (relative risk 0.39). 5

Discontinuation Protocol

When stopping PrEP, continue daily dosing for 7 days after the last at-risk sexual exposure. 3 This extended tail-off period is necessary due to the slower drug accumulation in vaginal tissue. 1

Critical Pitfalls to Avoid

  • Never use on-demand "2-1-1" dosing in women—this regimen is only validated for men who have sex with men. 1, 3
  • Do not assume protection before 7 days of daily dosing—counsel women about this critical lead-in period. 2, 3
  • PrEP does not prevent other STIs—condoms remain recommended for comprehensive STI prevention. 1, 3
  • Screen for acute HIV infection symptoms before initiation—starting PrEP during unrecognized acute HIV can lead to resistance. 1

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

HIV Pre-Exposure Prophylaxis (PrEP) Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

HIV Pre-Exposure Prophylaxis Recommendations

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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