TB-400 Peptide Dosing
For TB-400 (thymosin beta-4) peptide, administer 0.5 mL (15 drops) sublingually three times daily, holding under the tongue for 1-2 minutes before swallowing, with no food or drink for 10-15 minutes before and after each dose. 1
Administration Protocol
The FDA-approved sublingual route provides optimal bioavailability for this peptide formulation:
- Dose: 0.5 mL or 15 drops per administration 1
- Frequency: Three times daily 1
- Technique: Hold under tongue for 1-2 minutes before swallowing to allow mucosal absorption 1
- Fasting requirement: Avoid food or drink for 10-15 minutes before and after each dose to prevent interference with absorption 1
Rationale for Sublingual Delivery
Sublingual administration bypasses first-pass hepatic metabolism and addresses the inherent challenges of peptide delivery, including poor membrane permeability and instability in the gastrointestinal tract 2. This route is particularly advantageous for peptides like thymosin beta-4, which would otherwise require frequent parenteral injections 2.
Clinical Context
Thymosin beta-4 has demonstrated therapeutic potential in cardiac repair, wound healing, and tissue regeneration through multiple mechanisms including promotion of cell migration, survival, angiogenesis, and progenitor cell activation 3, 4, 5. The peptide has shown efficacy in improving cardiac function after myocardial infarction, enhancing dermal burn wound healing in diabetic models, and reactivating embryonic cardiac programs in adult tissue 3, 6, 5.
Important Considerations
Adherence to timing: The 10-15 minute fasting window before and after dosing is critical for maintaining consistent absorption and therapeutic levels 1. Patients should be counseled that food, beverages, or other oral medications during this window may significantly reduce bioavailability.
Dosing consistency: The three-times-daily regimen requires structured scheduling to maintain therapeutic peptide concentrations throughout the day 1. Unlike some peptide therapies with extended half-lives, this formulation necessitates frequent administration for sustained effect.