What are the normal ranges for glucose, proteins, pH, and lactate dehydrogenase (LDH) in cerebrospinal fluid (CSF)?

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Normal Ranges of CSF Parameters

The normal cerebrospinal fluid values are: glucose 2.6-4.5 mmol/L (47-81 mg/dL), protein <0.4 g/L, CSF/plasma glucose ratio >0.66, white blood cell count <5 cells/μL, and opening pressure 12-20 cm CSF. 1

Glucose

  • Normal CSF glucose ranges from 2.6 to 4.5 mmol/L (approximately 47-81 mg/dL) 1
  • CSF glucose is typically about two-thirds of the plasma glucose concentration 1
  • The CSF/plasma glucose ratio should normally be >0.66 1
  • CSF glucose levels are influenced by blood glucose levels up to 6 hours before lumbar puncture, so timing matters when interpreting isolated values 2

Clinical Context for Glucose Interpretation

  • A CSF glucose >2.6 mmol/L makes bacterial meningitis unlikely 1
  • In patients with normal glucose metabolism, the CSF/blood glucose ratio ranges from 0.35-0.95 at 0-6 hours before lumbar puncture 2
  • For patients with abnormal or unclear glucose metabolism, the CSF/average blood glucose ratio (0.33-0.78) should be used rather than absolute values 2

Protein

  • Normal CSF protein is <0.4 g/L 1
  • Local laboratory ranges should be consulted as they may vary slightly from these quoted values 1
  • A CSF protein <0.6 g/L makes bacterial meningitis unlikely 1

pH

  • Normal CSF pH is not explicitly defined in the provided guidelines, as pH measurement is not routinely performed in standard CSF analysis 1
  • The guidelines focus on lactate measurement rather than pH for distinguishing bacterial from viral meningitis 1

Lactate Dehydrogenase (LDH)

  • Normal CSF LDH values are not standardized in the major meningitis guidelines 1
  • One research study suggests a CSF LDH value of <40 U/L may be considered normal, with values ≥40 U/L showing 86.3% sensitivity for detecting bacterial meningitis 3
  • A CSF/serum LDH ratio of <0.1:1.0 is generally considered normal 3
  • LDH is not routinely recommended in current guidelines for CSF analysis, as lactate measurement has largely replaced it for diagnostic purposes 1

Additional Normal CSF Parameters

  • White blood cell count: <5 cells/μL 1
  • Opening pressure: 12-20 cm CSF (when measured in lateral recumbent position, not sitting) 1
  • Appearance: Clear 1
  • Predominant cell type: None (or rare lymphocytes if any cells present) 1

Important Clinical Caveats

  • A traumatic lumbar puncture will falsely elevate white cells and protein due to blood contamination; a common correction factor used is 1:1000 (1 WBC per 1000 RBCs) 1
  • Neonates have different normal values: term neonates may have higher CSF protein and different CSF/blood glucose ratios, with a ratio of 0.6 considered abnormal (not 0.4 as in older children and adults) 4
  • CSF lactate (not LDH) has become the preferred rapid test, with normal values <2 mmol/L effectively ruling out bacterial disease 5
  • When interpreting CSF glucose in isolation without simultaneous plasma glucose, values must be interpreted cautiously in the clinical context 1

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Cerebrospinal fluid lactate and lactate dehydrogenase activity in the rapid diagnosis of bacterial meningitis.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde, 1986

Guideline

CSF Glucose Ratio in Bacterial Meningitis Diagnosis

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Characteristic CSF Picture of Viral Meningitis

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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