What is the significance of a positive Lyme disease (Lyme) total antibody Chemiluminescent Immunoassay (CIA) when both Immunoglobulin G (IgG) and Immunoglobulin M (IgM) antibodies are negative?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: April 16, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

From the Guidelines

A negative Lyme total antibody CIA, along with negative Lyme IgG and IgM tests, strongly suggests that the patient does not have Lyme disease, as supported by the current guidelines for laboratory diagnosis of Lyme disease in the United States 1. When interpreting these test results, it's crucial to consider the patient's clinical presentation, history of tick exposure, and the timing of symptom onset. The 2-tiered serologic analysis, which includes an initial screening test like the CIA followed by a confirmatory Western immunoblot, is the recommended approach for diagnosing extracutaneous Lyme disease 1.

  • Key points to consider include:
    • The total antibody CIA detects both IgG and IgM antibodies, providing a comprehensive assessment of the immune response to Borrelia burgdorferi.
    • Negative results for all three tests (total antibody CIA, IgG, and IgM) make it unlikely that the patient has a current or past Lyme infection, with the exception of very early infections where antibodies may not have developed yet.
    • Clinical suspicion of Lyme disease should remain high if the patient presents with characteristic symptoms (e.g., erythema migrans, arthritis, neurologic manifestations) or a history of tick exposure, even with negative initial test results.
    • In such cases, repeating the tests in 2-4 weeks may be necessary to capture the development of antibodies, as the immune response can take time to mature.
    • It's also important to explore alternative diagnoses, as many conditions can mimic the symptoms of Lyme disease, ensuring that the patient receives appropriate care for their actual condition.

From the Research

Significance of Lyme Total Antibody CIA

  • The provided studies do not directly address the significance of a Lyme total antibody CIA when both the Lyme IgG and Lyme IgM are negative 2, 3, 4, 5, 6.
  • The studies focus on the diagnosis, treatment, and prevention of Lyme disease, as well as the efficacy and safety of various antibiotics for treating the disease 2, 3, 4, 5, 6.
  • None of the studies provide information on the interpretation of Lyme total antibody CIA results in the context of negative IgG and IgM results.
  • Therefore, there is no direct evidence to assist in answering this question, and further research or consultation with a medical professional may be necessary to understand the significance of this test result.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.