What is the recommended biweekly dose of testosterone cypionate for an adult male undergoing testosterone replacement therapy?

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Last updated: February 8, 2026View editorial policy

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Recommended Biweekly Dose of Testosterone Cypionate

For testosterone replacement therapy in adult males with confirmed hypogonadism, the recommended biweekly dose of testosterone cypionate is 100-200 mg administered intramuscularly every 2 weeks. 1, 2

Standard Dosing Guidelines

The FDA-approved dosing range for testosterone cypionate is 50-400 mg every 2-4 weeks, with the most common regimen being 100-200 mg every 2 weeks. 1, 2 The American College of Gastroenterology specifically recommends starting at 100-200 mg every 2 weeks or 50-100 mg weekly for testosterone enanthate or cypionate. 1

Dosing Frequency Considerations

  • Weekly dosing of 50-100 mg provides more stable testosterone levels compared to biweekly administration, which is increasingly preferred to minimize fluctuations. 1
  • Peak serum testosterone levels occur 2-5 days after injection, with levels often returning to baseline by 10-14 days post-injection. 1, 3
  • The biweekly 100-200 mg regimen creates significant peak-to-trough variation, spending considerable time in both supraphysiologic and subtherapeutic ranges. 1

Monitoring Requirements

Testosterone levels should be measured 2-3 months after treatment initiation or any dose change, drawn midway between injections (days 5-7), targeting mid-normal values of 450-600 ng/dL. 1, 3 Once stable levels are achieved, monitoring should occur every 6-12 months. 1

Critical Safety Monitoring

  • Hematocrit/hemoglobin must be monitored at each visit, as injectable testosterone causes erythrocytosis in approximately 43.8% of patients. 1 Treatment should be withheld if hematocrit exceeds 54%. 1
  • PSA and digital rectal examination should be performed in men over 40 years. 1
  • Injectable testosterone carries greater cardiovascular risk compared to transdermal preparations, possibly due to fluctuating testosterone levels. 1

Dose Adjustment Algorithm

If testosterone levels are below target (450-600 ng/dL) at midpoint testing and symptoms persist, increase the dose incrementally. 1 If levels exceed 1000 ng/dL, reduce the dose by approximately 50%. 1

When to Consider Weekly Dosing Instead

Patients experiencing significant mood or energy fluctuations between injections should be switched from biweekly 100-200 mg to weekly 50-100 mg dosing, as this provides more stable day-to-day testosterone levels and reduces the risk of erythrocytosis. 1 Weekly dosing is particularly important for patients with cardiovascular risk factors or those who develop hematocrit elevations on biweekly dosing. 1

Common Pitfalls to Avoid

  • Do not measure testosterone levels at peak (days 2-5) or trough (days 13-14), as this will lead to inappropriate dose adjustments. 1 Always measure midway between injections (days 5-7). 1
  • Do not continue biweekly dosing if the patient develops erythrocytosis (hematocrit >52%), as this significantly increases thrombotic risk. 1 Switch to weekly dosing or transdermal formulations. 1
  • Do not use testosterone therapy in men actively seeking fertility, as it causes azoospermia; gonadotropin therapy (hCG plus FSH) is mandatory instead. 1, 3

Alternative Formulation Considerations

If erythrocytosis develops on injectable testosterone (hematocrit 52-54%), switch to transdermal testosterone gel, which has a markedly lower erythrocytosis rate of 15.4% compared to 43.8% with injectables. 1 Transdermal formulations provide more stable testosterone levels and may be preferred as first-line therapy in elderly patients or those with cardiovascular disease. 1

References

Guideline

Testosterone Replacement Therapy Dosing

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

Testosterone Injection Treatment for Male Hypogonadism

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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