How should I evaluate a patient with suspected ankylosing spondylitis?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: February 9, 2026View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Workup for Ankylosing Spondylitis

Begin with plain radiographs of the sacroiliac joints (anteroposterior pelvis view) as your first-line imaging, and order HLA-B27 testing concurrently in patients with chronic back pain (≥3 months) that started before age 45 with inflammatory characteristics. 1, 2, 3

Initial Clinical Assessment

Target patients with these specific inflammatory back pain features, which have 75% sensitivity for axial spondyloarthritis 2, 3:

  • Insidious onset before age 40-45 years with symptoms lasting ≥3 months 2
  • Morning stiffness lasting >30 minutes that is prolonged 2, 3
  • Improvement with exercise but NOT with rest (key distinguishing feature) 2, 3
  • Night pain awakening in the second half of the night 2, 3
  • Alternating buttock pain indicating sacroiliac joint involvement 2, 3

Additional Clinical Features to Document

  • Peripheral arthritis: Large joint involvement (especially knees) in 30-50% of patients, typically oligoarticular and asymmetric 2
  • Extra-articular manifestations: Uveitis, psoriasis, or inflammatory bowel disease 2
  • Family history: Personal or family history of related spondyloarthropathies 4

Initial Laboratory and Imaging Workup

Laboratory Testing

  • HLA-B27: Present in 74-89% of AS patients, though NOT diagnostic alone 2, 3
    • The American Academy of Family Physicians explicitly states: do NOT rule out spondyloarthritis based solely on a negative HLA-B27 test 2
  • C-reactive protein (CRP): May be elevated but is NOT always elevated, so normal inflammatory markers do not exclude disease 2, 5

Initial Imaging

Radiography is the appropriate first-line imaging modality 1, 3:

  • Anteroposterior pelvis radiograph to evaluate sacroiliac joints (rated 9/9 appropriateness by ACR) 1, 3
  • Add cervical and lumbar spine radiographs if symptoms are referable to these areas 1, 3
  • Radiographs demonstrate chronic erosions, sclerotic changes, and ankylosis but have limited sensitivity (19-72%) for early disease 1, 3

Critical Pitfall with Radiography

Radiographs miss more than half of patients with structural changes compared to advanced imaging, and interobserver agreement is only fair to moderate 1, 3. One study showed 41.3% of radiography reports gave incorrect diagnoses 1. Do NOT stop at negative radiographs if clinical suspicion remains high 1, 3.

Next Step: MRI When Indicated

Order MRI of the sacroiliac joints (without contrast is sufficient) if 1, 3:

  • Radiographs are negative or equivocal BUT clinical suspicion remains high 1, 3
  • Short duration of symptoms (early disease suspected) 1, 3
  • MRI can detect inflammatory changes 3-7 years before radiographic findings appear 1, 3, 5

MRI of both sacroiliac joints AND spine may be considered for comprehensive evaluation, though sacroiliac joint MRI alone is often sufficient initially 1, 6

Imaging Modalities to AVOID

The ACR guidelines are explicit about what NOT to order 1:

  • CT: Not routinely obtained for initial evaluation 1
  • Bone scan with SPECT or SPECT/CT: No relevant literature supporting use 1
  • Fluoride PET/CT: Not routinely obtained 1
  • Ultrasound: Not a routine diagnostic tool, limited to superficial posterior margins 1

Diagnostic Algorithm Summary

  1. Screen clinically: Chronic back pain ≥3 months, onset <45 years, with inflammatory characteristics 2, 3
  2. Order concurrently: Plain radiographs (AP pelvis + spine if symptomatic) AND HLA-B27 2, 3
  3. If radiographs show sacroiliitis: Diagnosis established, refer to rheumatology 3
  4. If radiographs negative but high clinical suspicion: Proceed to MRI sacroiliac joints 1, 3
  5. Refer to rheumatology if positive HLA-B27 with high clinical suspicion OR if MRI shows inflammatory changes 2, 3

Why Early Diagnosis Matters

Diagnosis is frequently delayed by 4.9-8 years from symptom onset 2, yet early treatment with NSAIDs and TNF inhibitors is increasingly effective and may slow radiographic progression 3, 5, 7. This makes aggressive workup in appropriate patients clinically imperative despite the diagnostic challenges.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Axial Spondyloarthritis Clinical Features

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

Initial Work-up for Suspected Ankylosing Spondylitis

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Ankylosing spondylitis: an update.

Australian family physician, 2013

Research

Diagnosing ankylosing spondylitis.

The Journal of rheumatology. Supplement, 2006

Related Questions

What is the diagnostic approach and treatment for a patient presenting with symptoms of ankylosing spondylitis, including a history of arthritis, eye problems, skin conditions, or gastrointestinal issues?
Can a CT scan of the cervical spine be used to diagnose ankylosing spondylitis?
What is the initial work-up for a patient suspected of having ankylosing spondylitis?
What is the diagnosis and recommended treatment for a 32-year-old male smoker with progressive low back pain, morning stiffness, and limited mobility, who has anemia (low Hemoglobin), elevated Erythrocyte Sedimentation Rate (ESR), and C-Reactive Protein (CRP), and has not responded to non-steroidal anti-inflammatory drugs (NSAIDs)?
What are the diagnostic criteria and treatment options for a young to middle-aged male patient presenting with chronic back pain and stiffness, particularly in the morning or after periods of inactivity, with a potential family history of ankylosing spondylitis?
What is ketoconazole used for?
What is the appropriate emergency department management for this patient?
What are the latest findings on diagnostic criteria and treatment options for mast‑cell activation syndrome (MCAS)?
What is the first‑line treatment for a thrombosed arteriovenous graft in a hemodialysis patient?
What are the dosing schedule, indications, contraindications, precautions, common side effects, and alternative therapies for Emgality (galcanezumab) for preventive treatment of migraine in adults?
Can 2% ketoconazole cream be used to treat a fungal intertriginous rash between the breasts?

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.