What blood tests should be obtained before giving intravenous alteplase to an adult with acute ischemic stroke?

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Blood Tests Before IV Alteplase for Acute Ischemic Stroke

Only blood glucose measurement is mandatory before initiating IV alteplase—all other laboratory tests should be sent but must not delay treatment. 1

Mandatory Pre-Treatment Testing

  • Capillary or serum blood glucose must be checked immediately and hypoglycemia (glucose <60 mg/dL or 3.3 mmol/L) must be treated with IV dextrose before alteplase administration. 1
  • This is the single laboratory value that must be obtained and corrected prior to treatment in all patients. 1

Laboratory Tests That Should Be Sent (But Not Awaited)

The following tests should be ordered immediately upon arrival but treatment should proceed without waiting for results: 1

  • Complete blood count with platelet count 1
  • Serum electrolytes and creatinine 1
  • INR and partial thromboplastin time (aPTT) 1
  • Prothrombin time (PT) 1
  • Serum troponin 1
  • Electrocardiography 1

When You Must Wait for Coagulation Studies

Stop and wait for laboratory results only in these specific circumstances: 1

  • Known warfarin use - Must confirm INR ≤1.7 and PT <15 seconds before treatment 1
  • Known heparin use - Must confirm aPTT ≤40 seconds before treatment 1
  • Clinical suspicion of bleeding disorder or thrombocytopenia - Must confirm platelets ≥100,000/mm³ before treatment 1
  • Uncertain anticoagulation history - Must obtain coagulation studies before treatment 1

Critical Laboratory Contraindications

Do not administer alteplase if laboratory values reveal: 1

  • Platelets <100,000/mm³ 1
  • INR >1.7 1
  • aPTT >40 seconds 1
  • PT >15 seconds 1

Special Anticoagulation Considerations

  • For patients on direct oral anticoagulants (DOACs) - Do not administer alteplase unless specific factor Xa or thrombin assays are normal OR the patient has not received a dose for >48 hours with normal renal function. 1
  • For patients on low molecular weight heparin (LMWH) - Do not administer alteplase if treatment dose was given within the previous 24 hours. 1

Treatment Initiation Strategy

In patients without known anticoagulant use or bleeding concerns: 1

  • Begin alteplase immediately after CT confirms no hemorrhage and glucose is checked 1
  • Treatment can be initiated before platelet count is available but should be discontinued if platelets return <100,000/mm³ 1
  • Treatment can be initiated before coagulation studies are available but should be discontinued if INR >1.7 or PT is abnormally elevated 1

Common Pitfalls to Avoid

  • Never delay treatment to wait for a "complete workup" in patients without bleeding concerns or known anticoagulant use—time is brain tissue. 1
  • Do not confuse this with myocardial infarction protocols where more extensive laboratory testing may be required before thrombolysis. 2
  • Remember that electrocardiography and troponin are recommended to identify concomitant acute coronary syndrome but should not delay stroke treatment. 1

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Laboratory Testing Before Alteplase Administration in Acute Myocardial Infarction

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

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Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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