What is the significance of a culture result showing Mixed Gram Positive (GP)/Gram Negative (GN) flora, specifically GMXPN (Gram-positive and Gram-negative bacteria)?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: April 22, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

From the FDA Drug Label

Meropenem has been shown to be active against most isolates of the following microorganisms, both in vitro and in clinical infections Gram-positive bacteria Enterococcus faecalis (vancomycin-susceptible isolates only) Staphylococcus aureus (methicillin-susceptible isolates only) Streptococcus agalactiae Streptococcus pneumoniae (penicillin-susceptible isolates only) Streptococcus pyogenes Viridans group streptococci Gram-negative bacteria Escherichia coli Haemophilus influenzae Klebsiella pneumoniae Neisseria meningitidis Proteus mirabilis Pseudomonas aeruginosa Anaerobic bacteria Bacteroides fragilis Bacteroides thetaiotaomicron Peptostreptococcus species

The Meropenem drug is effective against Gram-positive and Gram-negative bacteria.

  • Gram-positive bacteria include:
    • Enterococcus faecalis
    • Staphylococcus aureus
    • Streptococcus agalactiae
    • Streptococcus pneumoniae
    • Streptococcus pyogenes
    • Viridans group streptococci
  • Gram-negative bacteria include:
    • Escherichia coli
    • Haemophilus influenzae
    • Klebsiella pneumoniae
    • Neisseria meningitidis
    • Proteus mirabilis
    • Pseudomonas aeruginosa
  • Anaerobic bacteria include:
    • Bacteroides fragilis
    • Bacteroides thetaiotaomicron
    • Peptostreptococcus species 1

From the Research

GMXPN Mixed Gram Positive/Gram Negative Flora should be treated with caution, and the combination of vancomycin and piperacillin/tazobactam should be avoided due to the increased risk of acute kidney injury, as shown in the most recent study 2. When treating GMXPN Mixed Gram Positive/Gram Negative Flora, it is essential to consider the clinical context, infection site, and patient factors.

  • For mild to moderate infections, empiric antibiotic therapy might include amoxicillin-clavulanate 875/125 mg twice daily for 7-10 days or ciprofloxacin 500 mg twice daily plus clindamycin 300-450 mg four times daily for 7-10 days to cover both gram-positive and gram-negative organisms.
  • For severe infections, broader coverage with meropenem 1 g IV every 8 hours may be necessary, as it has been shown to have a lower risk of nephrotoxicity compared to piperacillin-tazobactam when used with vancomycin 3, 4, 2. Key points to consider:
  • The presence of mixed flora in normally sterile sites always indicates infection requiring prompt treatment.
  • Source control through drainage or debridement is essential for abscesses or infected wounds.
  • Mixed flora can represent normal colonization in some body sites like the oral cavity, gastrointestinal tract, or skin, so clinical correlation is necessary to determine if treatment is warranted.
  • The most recent study 2 highlights the importance of avoiding the combination of vancomycin and piperacillin/tazobactam due to the increased risk of acute kidney injury, and instead recommends using alternative antibiotics such as cefepime or meropenem.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.