How should I reconstitute a 10 mg vial of tesamorelin with sterile water for injection using aseptic technique?

Reconstitution of Tesamorelin 10mg

Reconstitute tesamorelin 10mg by injecting 2.2 mL of sterile water for injection into the vial using strict aseptic technique, then gently roll (do not shake) the vial between your palms until the powder is completely dissolved.

Aseptic Technique Requirements

The reconstitution process must follow rigorous sterile procedures to prevent contamination:

• Perform all mixing in a clean environment using aseptic technique 1
• Thoroughly wash hands to wrists with detergent or soap and potable water before beginning 1
• Sanitize the rubber stopper of the tesamorelin vial with 70% isopropanol (alcohol) before needle puncture 1, 2
• Use only sterile needles and syringes, avoiding any direct contact contamination from touch, nonsterile objects, or other materials 1

Step-by-Step Reconstitution Process

Preparation Phase

• Gather sterile water for injection (preservative-free), sterile syringes, and sterile needles 1
• Remove the protective cap from the tesamorelin vial and wipe the rubber stopper with 70% alcohol 1, 2
• Allow the alcohol to dry completely before proceeding 1

Reconstitution Phase

• Draw up 2.2 mL of sterile water for injection using a sterile syringe 1
• Remove any air bubbles by holding the syringe upright and tapping the side to release bubbles to the top 1
• Inject the sterile water slowly into the tesamorelin vial, directing the stream against the glass wall rather than directly onto the powder 1
• Gently roll the vial between your palms—do NOT shake vigorously—until the powder is completely dissolved 1
• Perform visual inspection for physical integrity, clarity, and absence of particulate matter 1

Critical Safety Considerations

Contamination Prevention

• Discard the vial immediately if sterility is compromised at any point during reconstitution 2, 3
• Never reuse syringes or needles, even if only the needle is changed 2, 3
• Avoid touching the rubber stopper or needle tip during the entire process 1, 2

Storage and Timing

• Use reconstituted tesamorelin immediately after preparation when possible 1
• If storage is necessary, refrigerate at 4°C and use within the timeframe specified by the manufacturer 1
• Do not use if the solution appears turbid, contains particulate matter, or if the expiration date has passed 1

Common Pitfalls to Avoid

• Shaking the vial vigorously can denature the peptide and create excessive foaming—always roll gently 1
• Injecting water too rapidly or directly onto the powder can cause foaming and incomplete dissolution 1
• Failing to properly sanitize the rubber stopper is a major source of contamination 1, 2
• Touching the needle tip or rubber stopper after sanitization compromises sterility 1, 2