What are the common causes of vaginitis and their first‑line treatments?

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Vaginitis: Common Causes and First-Line Treatments

The three most common infectious causes of vaginitis—bacterial vaginosis (40-50% of cases), vulvovaginal candidiasis (20-25%), and trichomoniasis (15-20%)—require prompt diagnosis using office-based testing (vaginal pH, wet mount, KOH prep) and targeted antimicrobial therapy, with metronidazole as first-line for bacterial vaginosis and trichomoniasis, and topical or oral azoles for candidiasis. 1, 2

Bacterial Vaginosis (Most Common)

Pathophysiology

  • Bacterial vaginosis results from replacement of normal H₂O₂-producing Lactobacillus species with anaerobic overgrowth, including Gardnerella vaginalis, Prevotella, Mobiluncus, and Mycoplasma hominis. 1, 3
  • This represents dysbiosis rather than a sexually transmitted infection, though multiple sexual partners increase risk. 1, 3

Diagnosis: Amsel Criteria (3 of 4 Required)

  • Homogeneous, thin white or gray discharge 1, 4
  • Vaginal pH > 4.5 1, 4
  • Positive whiff test (fishy amine odor with 10% KOH) 1, 4
  • Clue cells on wet mount microscopy 1, 4

First-Line Treatment

  • Metronidazole 500 mg orally twice daily for 7 days is the CDC-recommended first-line therapy. 1, 4
  • Alternative regimens include intravaginal metronidazole gel or intravaginal clindamycin cream. 4, 2
  • Tinidazole is an alternative oral option for metronidazole-intolerant patients. 1

Critical Pitfalls

  • Up to 50% of women with bacterial vaginosis are asymptomatic, so screening may be warranted before gynecologic procedures. 3
  • Treating male partners does not prevent recurrence because this is not a classic STI. 1, 3
  • Recurrence rates reach 50-80% within one year due to failure of Lactobacillus recolonization. 3
  • In pregnancy, bacterial vaginosis is associated with preterm delivery and premature rupture of membranes; treatment with metronidazole is recommended. 4, 5

Vulvovaginal Candidiasis

Clinical Presentation

  • Thick, white "cottage cheese-like" discharge with intense vulvar pruritus is characteristic. 1
  • Vulvovaginal erythema and swelling are common findings. 4, 6
  • Vaginal pH remains normal (3.8-4.5), distinguishing it from bacterial vaginosis and trichomoniasis. 1, 7

Diagnosis

  • Microscopic examination with 10% KOH preparation revealing budding yeast or pseudohyphae confirms the diagnosis. 1, 7
  • Culture with speciation is crucial for recurrent or complicated cases to identify non-albicans species (C. glabrata, C. tropicalis). 8, 2

First-Line Treatment

  • Topical azole formulations (butoconazole, clotrimazole) or oral fluconazole are equally efficacious for uncomplicated infections. 4, 6
  • In pregnancy, only topical azoles are recommended; oral fluconazole should be avoided. 4, 2

Management of Complicated Cases

  • For Candida albicans recurrent infections, maintenance fluconazole therapy is effective. 8
  • For non-albicans Candida (especially C. glabrata), vaginal boric acid therapy is the first-choice treatment. 8

Trichomoniasis

Clinical Presentation

  • Frothy, greenish-yellow vaginal discharge with prominent vulvar itching is the hallmark. 1, 4
  • "Strawberry cervix" (punctate hemorrhagic lesions) may be visible on speculum examination. 1
  • Vaginal pH > 4.5 (often > 5.4) helps differentiate from candidiasis. 1, 7

Diagnosis

  • Wet mount microscopy showing motile flagellated trichomonads is diagnostic but has 30-50% false-negative rate. 1
  • Nucleic acid amplification testing (NAAT) is recommended due to superior sensitivity compared to wet mount. 4, 2

First-Line Treatment

  • Metronidazole 2 g orally as a single dose is the CDC-recommended first-line therapy. 1, 4
  • Tinidazole is an alternative for treatment failures or metronidazole-resistant cases. 1, 8

Critical Management Points

  • All sexual partners must be treated simultaneously with the same single-dose regimen to prevent reinfection. 1, 4
  • Patients should abstain from sexual intercourse until both partners complete therapy and are asymptomatic. 1, 4
  • In pregnancy, the same 2 g single-dose metronidazole regimen is safe and recommended due to associations with preterm delivery and premature rupture of membranes. 1, 4
  • Trichomoniasis increases HIV acquisition and transmission risk. 1

Essential Diagnostic Algorithm

Office-Based Testing (Required for All Cases)

  • Vaginal pH testing differentiates causes: normal (3.8-4.5) suggests candidiasis; elevated (>4.5) suggests bacterial vaginosis or trichomoniasis. 1, 7
  • Wet mount examination identifies motile trichomonads, clue cells (bacterial vaginosis), or budding yeast. 1, 4
  • KOH preparation reveals pseudohyphae (candidiasis) and produces fishy odor (positive whiff test) in bacterial vaginosis. 1, 4
  • Microscopic examination is essential; symptoms and physical examination alone are inadequate for accurate diagnosis. 1

Additional Testing When Indicated

  • NAAT for Trichomonas vaginalis, Chlamydia trachomatis, and Neisseria gonorrhoeae should be performed in high-risk patients or when cervicitis is suspected. 4, 2
  • Yeast culture with speciation is indicated for recurrent or treatment-refractory vulvovaginal candidiasis. 8, 2

Noninfectious Causes (5-10% of Cases)

Atrophic Vaginitis

  • Results from estrogen deficiency and presents with vaginal dryness, irritation, and dyspareunia. 6, 7
  • Topical or systemic estrogen therapy is effective treatment. 6, 2

Irritant/Allergic Vaginitis

  • Caused by chemical irritants or allergens; treatment involves identifying and removing the offending agent. 7, 2

References

Guideline

Vaginal Infections Diagnosis and Treatment

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Research

Vaginitis: Diagnosis and Treatment.

American family physician, 2018

Guideline

Bacterial Vaginosis Pathogenesis and Risk Factors

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Vaginitis Diagnosis and Treatment

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Research

Bacterial vaginosis.

Primary care update for Ob/Gyns, 2000

Research

Management of vaginitis.

American family physician, 2004

Research

Vaginitis.

American family physician, 2011

Research

Management of persistent vaginitis.

Obstetrics and gynecology, 2014

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Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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