HGH Reconstitution Volume
Add 1 mL of bacteriostatic water to a 10 IU vial of HGH to achieve a standard concentration of 10 IU/mL, which provides optimal dosing precision and stability for subcutaneous administration.
Reconstitution Rationale
The volume of bacteriostatic water used for reconstitution should be based on achieving a practical concentration that balances injection volume, dosing accuracy, and solution stability:
Standard concentration approach: Using 1 mL of bacteriostatic water for a 10 IU vial creates a 10 IU/mL solution, allowing for straightforward dosing where each 0.1 mL (10 units on an insulin syringe) delivers 1 IU of HGH 1
Alternative concentrations: You may use 2 mL of bacteriostatic water to create a 5 IU/mL solution if larger injection volumes are acceptable, or 0.5 mL for a 20 IU/mL concentration if minimizing injection volume is critical 1
Proper Reconstitution Technique
Follow strict aseptic technique to prevent contamination and preserve drug integrity:
Sanitize the vial stopper with 70% isopropanol before needle insertion to prevent microbial contamination 2
Inject the bacteriostatic water slowly down the side of the vial rather than directly onto the powder to minimize foaming and protein denaturation 2
Gently swirl the vial in a circular motion until the powder is completely dissolved—never shake vigorously, as this can damage the protein structure of HGH 2
Visually inspect the reconstituted solution for clarity and absence of particulate matter before use; discard if discolored or cloudy 2, 3
Storage and Stability Considerations
Proper storage is essential for maintaining HGH potency after reconstitution:
Refrigerate immediately at 2-8°C (36-46°F) after reconstitution 2
Multi-dose stability: Bacteriostatic water contains approximately 0.9% benzyl alcohol as a preservative, permitting multi-dose use for up to 28 days when refrigerated 2, 1
Label the vial with the reconstitution date, concentration, and beyond-use date to prevent dosing errors 2
Critical Safety Points
Avoid common reconstitution errors that compromise drug safety and efficacy:
Never use sterile water for injection (without preservative) for multi-dose vials, as this significantly increases contamination risk 2
Do not use expired or moisture-exposed powder that appears discolored or clumped before reconstitution 2
Discard any reconstituted solution that develops discoloration, cloudiness, or visible particles during storage 2, 3
Maintain sterile technique throughout the process, avoiding contact of needles or syringes with non-sterile surfaces 2