What testing is indicated in the setting of acute alopecia (hair loss)?

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From the Guidelines

In the setting of acute hair loss, initial testing should include a complete blood count (CBC), thyroid function tests (TSH, free T4), and a comprehensive metabolic panel, as these tests help identify common causes like thyroid disorders and nutritional deficiencies. Additional testing may include antinuclear antibody (ANA) testing for autoimmune conditions, vitamin D levels, and hormone panels (testosterone, DHEAS) particularly in women with signs of androgen excess 1.

Key Considerations

  • A detailed medical history is essential, focusing on recent illnesses, medications, stressors, and family history of hair disorders.
  • Specific testing should be guided by the clinical presentation - for example, fungal cultures for suspected tinea capitis or a pull test to assess active shedding.
  • Iron studies (serum iron, ferritin, total iron binding capacity) may not be necessary as the evidence for an association between iron deficiency and alopecia areata is not strong, with some studies finding no differences in iron status between patients with alopecia areata and controls 1.
  • A scalp biopsy may be necessary if the diagnosis remains unclear after initial testing.

Diagnostic Approach

  • The diagnosis of alopecia areata is usually straightforward, but can be confused with other conditions such as trichotillomania, tinea capitis, and telogen effluvium 1.
  • Dermoscopy can aid in the diagnosis of alopecia areata, highlighting features such as dystrophic hairs with fractured tips (exclamation mark hairs) and hairs fractured before emergence from the scalp (cadaverized hairs) 1.
  • The clinical presentation and medical history should guide the diagnostic approach, with a focus on identifying underlying conditions that may be contributing to the hair loss.

From the FDA Drug Label

The FDA drug label does not answer the question.

From the Research

Testing for Acute Hair Loss

In the setting of acute hair loss, several tests can be performed to determine the underlying cause.

  • A comprehensive history and physical examination are essential in evaluating hair loss, as they can help identify potential causes such as malnutrition, autoimmune diseases, and endocrine disorders 2.
  • Targeted laboratory testing may include:
    • Complete blood count
    • Ferritin
    • Thyroid stimulating hormone (TSH)
    • Vitamin D 25OH 3
  • Scalp biopsy can confirm a diagnosis, especially in cases of cicatricial alopecia 3.
  • Hair pull, hair tug, Hair Card, and hair mount can aid in evaluating the hair and scalp 3.
  • Thyroid profile testing may be relevant in certain types of alopecia, such as alopecia areata, which has been associated with thyroid dysfunction 4, 5.

Specific Tests for Different Types of Alopecia

  • For alopecia areata, testing for thyroid dysfunction, such as TSH and antithyroid antibodies, may be considered, especially if the patient has a personal or family history of autoimmunity 4, 5.
  • For telogen effluvium, thyroid hormone assays may be performed to rule out underlying thyroid anomalies 5.
  • For frontal fibrosing alopecia, thyroid testing may be considered due to the higher prevalence of thyroid conditions in this population 5.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Hair Loss: Diagnosis and Treatment.

American family physician, 2024

Research

How to diagnose hair loss.

Dermatologic clinics, 2013

Research

Study of the Thyroid Profile of Patients with Alopecia.

Journal of clinical medicine, 2023

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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