What are the benefits of methylene blue (methylthioninium chloride)?

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Last updated: February 25, 2025View editorial policy

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From the Guidelines

Methylene blue is a highly effective treatment for methemoglobinemia, and its benefits include reducing MetHb levels, with a usual starting dose of 1–2 mg/kg infused intravenously over 3 to 5 minutes 1. The primary use of methylene blue is for the treatment of methemoglobinemia, a condition characterized by elevated levels of methemoglobin in the blood. Methylene blue acts by accepting an electron from NADPH, reducing the 3+ ferric state back to the 2+ ferrous state in erythrocytes.

  • The usual starting dose is 1–2 mg/kg (0.2 mL/kg of a 1% solution) infused intravenously over 3 to 5 minutes 1.
  • The dose may be repeated at 1 mg/kg if methemoglobinemia does not significantly decrease within 30–60 minutes 1.
  • Methylene blue should reduce MetHb levels significantly in less than an hour 1. However, methylene blue should be used with caution in certain patient populations, such as those with G6PD deficiency, as it may induce hemolysis and worsen methemoglobinemia 1.
  • Patients with G6PD deficiency may not produce sufficient NADPH to reduce methylene blue to leukomethylene blue, making methylene blue therapy ineffective 1.
  • Methylene blue should be avoided in patients with G6PD deficiency, and ideally, all patients should be tested for G6PD deficiency before methylene blue administration 1. Additionally, methylene blue may interact dangerously with serotonergic medications like SSRIs, potentially causing serotonin syndrome 1.
  • Methylene blue should be used cautiously in patients with renal failure and in anesthetized patients, as it may inhibit guanylate cyclase, decreasing nitric oxide-mediated vasodilatation leading to systemic and pulmonary hypertension 1. In terms of other potential benefits, methylene blue has been studied for its neuroprotective effects, antioxidant properties, and antimicrobial applications, but these uses are still being researched and require further study 1.
  • Methylene blue may have potential benefits for Alzheimer's disease and other neurodegenerative conditions, but more research is needed to confirm its efficacy 1.
  • Methylene blue demonstrates antioxidant properties by acting as an electron donor in the mitochondrial electron transport chain, improving cellular energy production at low doses (1-2 mg/kg) 1. Overall, methylene blue is a valuable treatment for methemoglobinemia, but its use requires careful consideration of potential risks and benefits, particularly in certain patient populations.

From the FDA Drug Label

The FDA drug label does not answer the question.

From the Research

Benefits of Methylene Blue

The benefits of methylene blue (methylthioninium chloride) are varied and have been documented in several studies. Some of the benefits include:

  • Antidepressant, anxiolytic, and neuroprotective properties, as documented by both animal and human studies 2
  • Stabilizing effect on mitochondrial function and dose-dependent effect on the generation of reactive oxygen species, which is of significant heuristic value 2
  • Potential treatment of organic/neurodegenerative disorders and as a neuroprotective agent in general 2
  • Successful treatment of psychotic and mood disorders, as well as a memory enhancer in fear-extinction training 2
  • Promising results in the treatment of bipolar disorder, producing an antidepressant and anxiolytic effect without risk of a switch into mania 2
  • Long-term use in bipolar disorder leading to better stabilization and a reduction in residual symptoms of the illness 2
  • Anti-aging properties, with the potential to bypass Complex I/III activity in mitochondria and diminish oxidative stress to some degree 3
  • Treatment of age-related conditions, including neurodegeneration, memory loss, skin aging, and premature aging disease, progeria 3

Therapeutic Uses

Methylene blue has been used in various therapeutic settings, including:

  • Additional blood pressure support in patients who develop severe, refractory vasoplegia 4
  • Treatment of ifosfamide-induced encephalopathy (IIE) 5
  • Cardiothoracic surgery 6
  • Treatment of methemoglobinemia and malaria

Important Considerations

It is essential to note that methylene blue can induce serotonin syndrome, especially when used in conjunction with other serotonergic agents 4, 6, 5. Therefore, caution is needed when administering methylene blue, particularly in patients taking serotonin-modulating agents.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Suspected serotonin syndrome in a patient being treated with methylene blue for ifosfamide encephalopathy.

Journal of oncology pharmacy practice : official publication of the International Society of Oncology Pharmacy Practitioners, 2012

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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