What blood tests are typically included in sexually transmitted disease (STD) testing through a blood draw?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: August 20, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Standard Blood Tests for Sexually Transmitted Disease Screening

Blood tests for STD screening typically include tests for HIV, syphilis, and hepatitis B, which are the primary sexually transmitted infections that can be reliably detected through blood testing. 1

Core Blood Tests for STD Screening

HIV Testing

  • Initial screening: Enzyme-linked immunosorbent assay (ELISA) or rapid assay
  • Confirmatory test: Western blot or other supplemental test if initial screening is positive
  • Important considerations:
    • HIV antibodies are detectable in ≥95% of patients within 6 months of infection
    • Negative tests cannot rule out infections acquired within the past 6 months
    • Informed consent must be obtained before testing 1

Syphilis Testing

  • Screening test: Serologic testing using Rapid Plasma Reagin (RPR) or similar test
  • Confirmatory test: If screening is positive, confirmatory testing is required
  • Testing frequency: Annual testing for those at risk, with more frequent testing (every 3-6 months) for high-risk individuals 1

Hepatitis B Testing

  • Standard tests: Hepatitis B surface antigen (HBsAg)
  • Additional markers: May include hepatitis B core antibody and surface antibody tests to determine infection status or immunity
  • Testing frequency: Annual testing for individuals at risk for STIs, with more frequent testing (every 3-6 months) for high-risk individuals, particularly injection drug users and men who have sex with men 2

Additional Considerations

Testing Frequency

  • Annual testing is recommended for individuals at ongoing risk
  • More frequent testing (every 3-6 months) is recommended for high-risk individuals 2

Special Populations

Pregnant Women

Pregnant women should receive comprehensive STD screening including:

  • Serologic test for syphilis during early pregnancy
  • Hepatitis B surface antigen testing
  • HIV testing, particularly for those with risk factors 1

High-Risk Individuals

For individuals at higher risk (multiple partners, men who have sex with men, injection drug users):

  • More frequent testing is recommended
  • Additional testing sites (pharyngeal, rectal) may be needed for comprehensive screening 3, 4

Common Pitfalls to Avoid

  • Inadequate follow-up: Ensuring proper linkage to care for positive results is essential
  • Incomplete testing: Not testing all relevant anatomical sites in high-risk individuals
  • Infrequent testing: Not repeating testing in high-risk individuals even when initial results are negative 2
  • Missing the window period: Failing to recognize that negative tests cannot rule out recent infections

Emerging Testing Options

  • Combined HIV and syphilis tests using a single finger prick blood sample are under evaluation
  • Self-collection options are becoming more available, which may increase testing rates 5, 6

Blood testing is only one component of comprehensive STD screening. For complete STD screening, additional tests including urine samples and swabs from other sites (pharyngeal, rectal) may be necessary depending on sexual practices and risk factors.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.