What is the recommended workup for starting testosterone (testosterone) therapy?

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Last updated: October 9, 2025View editorial policy

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Recommended Workup for Starting Testosterone Therapy

Prior to initiating testosterone therapy, clinicians should perform a comprehensive evaluation including hemoglobin/hematocrit, PSA (for men over 40), and assessment of cardiovascular risk factors to ensure safe and effective treatment. 1

Initial Laboratory and Clinical Assessment

  • Measure morning total testosterone levels between 8-10 AM on at least two separate occasions to confirm testosterone deficiency 1
  • Measure free testosterone by equilibrium dialysis and sex hormone-binding globulin, particularly in obese patients 1
  • Measure luteinizing hormone (LH) and follicle-stimulating hormone (FSH) to distinguish between primary and secondary hypogonadism 1
  • Obtain baseline hemoglobin/hematocrit (withhold therapy if hematocrit exceeds 50%) 1
  • Measure PSA in men over 40 years of age to exclude occult prostate cancer 1
  • Assess for cardiovascular disease risk factors (both fixed and modifiable) 1

Fertility Considerations

  • Perform testicular examination to evaluate size, consistency, and descent 1
  • Measure serum FSH to assess underlying reproductive health status 1
  • Consider semen analysis for patients interested in preserving fertility 1
  • Offer reproductive genetic testing (karyotype and Y-chromosome microdeletion analysis) for patients with severe oligospermia (<5 million sperm/mL) or non-obstructive azoospermia 1
  • Counsel patients that testosterone therapy will impair spermatogenesis and should not be used in men actively trying to conceive 1

Additional Evaluations Based on Clinical Presentation

  • For patients with low testosterone and low LH/FSH (secondary hypogonadism), consider measuring serum prolactin and iron saturation 1
  • Consider pituitary function testing and MRI of the sella turcica in cases of secondary hypogonadism 1
  • For patients with history of cardiovascular events, consider delaying testosterone therapy for 3-6 months after the most recent event 1

Patient Counseling Prior to Initiation

  • Discuss potential benefits: improvements in erectile function, libido, anemia, bone mineral density, lean body mass, and depressive symptoms 1
  • Inform about potential risks: polycythemia (especially with injectable forms), sleep apnea exacerbation, and effects on fertility 1
  • Counsel that current evidence cannot definitively state whether testosterone therapy increases or decreases cardiovascular risk 1
  • Advise on lifestyle modifications (weight loss, physical activity) as adjunctive treatment 1
  • Discuss the variable time course for recovery of spermatogenesis after cessation of therapy for patients interested in future fertility 1

Common Pitfalls and Caveats

  • Avoid alkylated oral testosterone preparations due to hepatotoxicity risk 1
  • Be aware that injectable testosterone is associated with the greatest treatment-induced increases in hemoglobin/hematocrit 1
  • For patients with elevated PSA at baseline, obtain a second PSA test to rule out spurious elevation before proceeding 1
  • Monitor for polycythemia during treatment; hematocrit >54% warrants dose reduction or temporary discontinuation 1
  • Recognize that testosterone therapy should target levels in the middle tertile of the normal reference range (450-600 ng/dL) 1

Following this comprehensive workup will help ensure appropriate patient selection for testosterone therapy while minimizing potential risks and optimizing treatment outcomes.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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