What is the workup for multiple myeloma?

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Last updated: February 12, 2025View editorial policy

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From the Guidelines

The workup for multiple myeloma should include a comprehensive initial diagnostic evaluation, comprising history and physical examination, as well as baseline blood studies and biological assessments to differentiate between symptomatic and asymptomatic multiple myeloma.

Initial Diagnostic Workup

  • Blood studies:
    • Complete Blood Count (CBC) with differential and platelet counts
    • Blood urea nitrogen (BUN)
    • Serum creatinine and serum electrolytes
    • Serum calcium
    • Albumin
    • Lactate dehydrogenase (LDH)
    • Beta-2 microglobulin
  • Serum and urine analyses:
    • Quantitative immunoglobulin levels (IgG, IgA, and IgM)
    • Serum protein electrophoresis (SPEP)
    • Serum immunofixation electrophoresis (SIFE)
    • Urine protein electrophoresis (UPEP)
    • Urine immunofixation electrophoresis (UIFE)
    • 24-hour urine for total protein
  • Serum free light chain (FLC) assay: useful for diagnosing and monitoring nonsecretory myeloma and light chain amyloidosis, and for documenting stringent complete response according to the International Myeloma Working Group Uniform Response Criteria 1

Bone Marrow and Imaging Studies

  • Bone marrow aspiration and biopsy: to detect quantitative and/or qualitative abnormalities of bone marrow plasma cells
  • Imaging studies:
    • Full skeleton radiographic survey or whole-body, low-dose CT to evaluate lytic bone lesions
    • Optional magnetic resonance imaging (MRI) for greater detail, especially if spinal cord compression is suspected

Cytogenetic and Molecular Studies

  • Chromosome analysis: using conventional karyotyping (cytogenetics) and fluorescence in situ hybridization (FISH) to detect chromosomal abnormalities, such as deletions, translocations, or amplifications
  • Specific chromosomal abnormalities: including deletion of 17p13, t(4;14), t(14;16), and t(11;14), which have prognostic implications 1 These studies are essential for establishing a diagnosis, assessing disease severity, and guiding treatment decisions in patients with multiple myeloma 1.

From the Research

Diagnostic Workup for Multiple Myeloma

The diagnostic workup for multiple myeloma includes:

  • Measurement of hemoglobin, serum creatinine, serum calcium, and serum free light chain levels 2
  • Serum protein electrophoresis with immunofixation 2, 3
  • 24-hour urine protein electrophoresis 2
  • Full-body skeletal imaging with computed tomography, positron emission tomography, or magnetic resonance imaging 2
  • Complete blood count with differential 3
  • Serum chemistries; creatinine, lactate dehydrogenase, and beta2-microglobulin tests 3
  • Immunoglobulin studies 3
  • Skeletal survey 3
  • Bone marrow evaluation 3

Staging and Prognosis

The Revised International Staging System combines data from serum biomarkers and malignant plasma cell genomic features to assess estimated progression-free survival and overall survival 2

  • The system uses serum biomarkers such as β2 microglobulin, albumin, and lactate dehydrogenase, as well as genomic features found on fluorescence in situ hybridization, such as t(4;14), del(17p), and t(14;16) 2

Treatment Approaches

Treatment approaches for multiple myeloma include:

  • Induction therapy with a combination of an injectable proteasome inhibitor, an oral immunomodulatory agent, and dexamethasone 2
  • Autologous hematopoietic stem cell transplantation followed by maintenance lenalidomide 2
  • Thromboprophylaxis, bisphosphonate therapy, and prophylaxis against infection 3
  • Management of issues related to pain, nutrition, and psychosocial support 3

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Multiple Myeloma: Diagnosis and Treatment.

American family physician, 2017

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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