What is the recommended dose of pyridoxine (Vitamin B6) with Anti-Tuberculosis Treatment (ATT)?

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Pyridoxine Dosing with Anti-Tuberculosis Treatment

The recommended dose of pyridoxine (vitamin B6) with isoniazid-containing anti-tuberculosis treatment is 25-50 mg daily for prophylaxis in at-risk patients, and 100 mg daily for treatment of established peripheral neuropathy. 1

Standard Prophylactic Dosing

For patients at risk of neuropathy, administer pyridoxine 25-50 mg daily throughout the entire course of isoniazid therapy. 1

High-Risk Populations Requiring Prophylaxis

Pyridoxine supplementation is mandatory for the following groups receiving isoniazid: 1

  • Pregnant women and breastfeeding infants
  • HIV-infected persons
  • Patients with diabetes mellitus
  • Patients with alcoholism
  • Malnourished patients
  • Patients with chronic renal failure
  • Elderly patients (advanced age)
  • Patients on other neurotoxic medications

The CDC specifically recommends this 25-50 mg/day prophylactic dose for patients taking tuberculosis medications, emphasizing that it should only be increased to 100 mg/day if peripheral neuropathy actually develops. 2

Therapeutic Dosing for Established Neuropathy

If peripheral neuropathy develops despite prophylaxis or in patients not receiving prophylaxis, increase pyridoxine to 100 mg daily. 1

This higher therapeutic dose has been shown to reverse isoniazid-induced neuropathy in multiple case reports, with clinical improvement occurring within weeks to months. 3, 4 In severe cases with motor-dominant neuropathy, high-dose vitamin B6 injections can reduce long-term morbidity. 4

Clinical Context and Mechanism

Isoniazid causes pyridoxine deficiency by interfering with pyridoxal phosphate (the active coenzyme form), leading to peripheral neuropathy that typically manifests as burning feet and distal sensory impairment. 3, 5 The risk is substantially higher in slow acetylators of isoniazid, who may require dose reduction to 2.5-3 mg/kg/day in addition to pyridoxine supplementation. 3, 6

Historical studies from the Tuberculosis Chemotherapy Centre demonstrated that even the low prophylactic dose of 6 mg daily was effective in preventing neuropathy with high-dose isoniazid therapy (12.5-15.6 mg/kg), making the currently recommended 25-50 mg daily dose more than adequate for prevention. 7, 5

Important Caveats

  • Do not exceed 100 mg/day for routine supplementation, as the European Food Safety Authority sets the upper tolerable intake level at 100 mg/day for adults, and higher doses can paradoxically cause sensory neuropathy. 2

  • Continue pyridoxine throughout the entire duration of isoniazid therapy, not just during the intensive phase. 1

  • In pregnancy, prophylactic pyridoxine 10 mg/day is specifically recommended along with standard anti-tuberculosis treatment, though the standard 25-50 mg dose is also safe. 6

  • Monitor for neuropathy development even with prophylaxis, particularly in slow acetylators, as this may indicate need for isoniazid dose reduction in addition to increased pyridoxine. 3

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Vitamin B6 Toxicity Management

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

[Isoniazid induced neuropathy: consider prevention].

Revue des maladies respiratoires, 2006

Research

Isoniazid induced motor-dominant neuropathy.

JPMA. The Journal of the Pakistan Medical Association, 2015

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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