What are the clinical trials on semaglutide (Glucagon-Like Peptide-1 receptor agonist)?

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Last updated: November 19, 2025View editorial policy

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Clinical Trials on Semaglutide

Major Cardiovascular Outcomes Trials

The SUSTAIN-6 trial is the pivotal cardiovascular outcomes study for subcutaneous semaglutide, demonstrating a 26% reduction in major adverse cardiovascular events (MACE) in patients with type 2 diabetes and established cardiovascular disease. 1

SUSTAIN-6 Trial Design and Results

  • Study population: 3,297 patients with type 2 diabetes randomized to once-weekly subcutaneous semaglutide (0.5 mg or 1.0 mg) or placebo for 2 years 1
  • Primary outcome: First occurrence of cardiovascular death, nonfatal MI, or nonfatal stroke occurred in 6.6% of the semaglutide group versus 8.9% in the placebo group (HR 0.74; 95% CI 0.58–0.95; P < 0.001) 1, 2
  • Key limitation: This was a moderate-sized trial powered for noninferiority testing for regulatory approval, not specifically designed as a cardiovascular outcomes trial 1
  • Discontinuations: More patients stopped treatment in the semaglutide group due to adverse events, predominantly gastrointestinal 1

PIONEER-6 Trial (Oral Semaglutide)

  • Study design: 3,183 patients with type 2 diabetes and high cardiovascular risk followed for a median of 15.9 months 1
  • Primary outcome: Oral semaglutide was noninferior to placebo for the composite outcome of cardiovascular death, nonfatal MI, or nonfatal stroke (HR 0.79; 95% CI 0.57–1.11; P < 0.001 for noninferiority) 1
  • Purpose: Preapproval trial designed to rule out unacceptable cardiovascular risk, not to demonstrate superiority 1
  • Future studies: The cardiovascular effects of oral semaglutide will be further evaluated in larger, longer-term outcomes trials 1

SUSTAIN Glycemic Control Trials (SUSTAIN 1-7)

The SUSTAIN clinical trial program evaluated semaglutide in over 8,000 patients across the spectrum of type 2 diabetes, consistently demonstrating superior glycemic control and weight loss versus all comparators. 3, 4

Key Efficacy Findings Across SUSTAIN Trials

  • HbA1c reduction: Semaglutide produced 1.5-1.9% HbA1c reduction after 30-56 weeks of treatment 5
  • Weight loss: Resulted in 5-10% weight reduction from baseline in clinical efficacy studies 5
  • Comparators evaluated: Placebo, sitagliptin, exenatide extended-release, and insulin glargine as monotherapy or add-on therapy 4, 5
  • Mechanism: Works via the incretin pathway, stimulating insulin and inhibiting glucagon secretion, while also decreasing energy intake by reducing appetite and food cravings 4

Pharmacokinetic Studies

Dosing and Administration Characteristics

  • Half-life: 7 days at doses of 0.5 or 1 mg, reaching steady state in 4-5 weeks 5, 6
  • Bioavailability: 89% absolute bioavailability with subcutaneous administration 6
  • Peak concentration: Reached 1-3 days post-dose 6
  • Steady-state concentrations: Approximately 65.0 ng/mL for 0.5 mg and 123.0 ng/mL for 1 mg once-weekly dosing 6
  • Elimination: Approximately 1-week half-life means semaglutide remains in circulation for about 5 weeks after the last dose 6

Special Populations

  • Renal impairment: Does not impact pharmacokinetics in a clinically relevant manner across all degrees of renal impairment (mild, moderate, severe, ESRD) 6
  • Hepatic impairment: No impact on semaglutide exposure across all degrees of hepatic impairment 6
  • No dose adjustments needed: Based on age, sex, race, ethnicity, or renal function 6

High-Dose Semaglutide Trial

A phase 2 trial evaluated semaglutide doses up to 16 mg weekly in 245 individuals with type 2 diabetes and BMI ≥27 kg/m², showing modest additional glucose-lowering with more weight loss but increased adverse events. 7

High-Dose Trial Results (40 weeks)

  • HbA1c change: Estimated treatment difference between 16 mg and 2 mg was -0.5 percentage points (95% CI -1.0 to -0.1; P = 0.015) for the hypothetical estimand 7
  • Weight change: -4.5 kg difference (95% CI -7.6 to -1.4; P = 0.004) favoring the 16 mg dose 7
  • Safety trade-off: Treatment-emergent adverse events and discontinuations due to adverse events (primarily gastrointestinal) were more frequent in the 8 mg and 16 mg groups compared to 2 mg 7
  • Hypoglycemia: No severe hypoglycemic episodes reported 7

Drug Interaction Studies

Semaglutide has minimal clinically relevant drug interactions, with no dose adjustments required for co-administered oral medications. 6

Evaluated Co-Medications

  • No significant interactions with metformin, oral contraceptives (ethinylestradiol/levonorgestrel), warfarin, digoxin, or atorvastatin 6
  • Gastric emptying delay: May influence absorption of concomitantly administered oral medications, though clinical studies showed no clinically relevant impact 6
  • CYP enzymes: Very low potential to inhibit or induce CYP enzymes or inhibit drug transporters 6

Safety Profile Across Trials

Common Adverse Events

  • Gastrointestinal effects: Most common reason for treatment discontinuation across all trials 1, 4
  • Hypoglycemia risk: Increased when combined with sulfonylureas (documented symptomatic hypoglycemia in 17.3-24.4% with semaglutide plus sulfonylurea) or insulin 6
  • Injection site reactions: Reported in 0.2% of semaglutide-treated patients 6
  • Pancreatic enzymes: Mean increase from baseline in amylase of 13% and lipase of 22% 6
  • Cholelithiasis: Reported in 1.5% (0.5 mg) and 0.4% (1 mg) of patients versus 0% with placebo 6
  • Heart rate: Mean increase of 2-3 beats per minute 6

Cardiovascular Safety

  • QTc interval: Does not prolong QTc intervals to any clinically relevant extent at 1.5 times the maximum recommended dose 6
  • Thyroid C-cell tumors: Carcinogenicity studies in mice and rats showed statistically significant increases in thyroid C-cell adenomas and carcinomas at all dose levels tested 6

Clinical Positioning Based on Guidelines

The American Diabetes Association and European Society of Cardiology strongly recommend semaglutide as part of the glucose-lowering regimen for patients with type 2 diabetes who have established atherosclerotic cardiovascular disease or are at high cardiovascular risk. 2

Recommended Dosing Strategy

  • Subcutaneous semaglutide: Start at 0.25 mg weekly and titrate to 0.5 mg or 1 mg weekly as tolerated 2
  • Titration rationale: Minimize gastrointestinal side effects by initiating at the lowest dose and following labeling instructions 2

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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