What is the significance of a Vitamin B12 (Vitamin B12) level of 230 pg/mL and what are the recommended treatment options?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: November 29, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Vitamin B12 Level of 230 pg/mL: Significance and Treatment

A Vitamin B12 level of 230 pg/mL represents metabolic B12 deficiency and warrants treatment, as this falls below the critical threshold of 258 pmol/L (approximately 350 pg/mL) where functional deficiency occurs even with "normal" laboratory ranges. 1, 2

Understanding This Level

Your B12 level of 230 pg/mL is concerning for several reasons:

  • This level falls in the "gray zone" where standard laboratory reference ranges may report it as "normal," but metabolic deficiency is highly likely 2, 3
  • Serum B12 <258 pmol/L (~350 pg/mL) with elevated homocysteine or methylmalonic acid (MMA) defines metabolic B12 deficiency, which affects 10.6% of patients overall and 18.1% of those over 80 years 1, 2
  • Up to 50% of patients with "normal" serum B12 have metabolic deficiency when measured by more sensitive markers like MMA 2
  • The UK NDNS defines deficiency as <150 pmol/L (~203 pg/mL), making your level of 230 pg/mL clearly deficient by this standard 2

Critical Next Steps for Diagnosis

You need additional testing to confirm functional deficiency:

  • Measure methylmalonic acid (MMA) - this is the most sensitive marker of functional B12 status and should be checked when B12 levels are indeterminate 1, 2, 3
  • Measure homocysteine levels - elevated levels suggest functional deficiency and increased stroke risk (target <10 µmol/L optimal) 1
  • Check complete blood count for megaloblastic anemia, though this may be absent in one-third of cases 2
  • Consider active B12 (holotranscobalamin) if available - this measures biologically active B12 and is more sensitive than total B12 2, 4

Recommended Treatment Approach

Start oral vitamin B12 supplementation at 1000-2000 mcg daily, which is as effective as intramuscular administration for most patients and costs less 2, 5, 6

Treatment Algorithm:

For patients WITHOUT severe neurologic symptoms:

  • Oral B12: 1000-2000 mcg daily until levels normalize, then maintenance therapy 2, 5
  • Continue until B12 levels are >350 pg/mL and MMA/homocysteine normalize 2
  • Monthly maintenance dosing after normalization 2

For patients WITH severe neurologic manifestations:

  • Intramuscular B12: 100 mcg daily for 6-7 days 7
  • Then 100 mcg on alternate days for seven doses 7
  • Then every 3-4 days for 2-3 weeks 7
  • Followed by 100 mcg monthly for life 7
  • Use methylcobalamin or hydroxycobalamin instead of cyanocobalamin when possible 1

Why This Matters for Your Health

Neurologic symptoms often present BEFORE hematologic changes and can become irreversible if untreated:

  • Cognitive difficulties, memory problems, concentration issues 2
  • Peripheral neuropathy 2, 5
  • Fatigue 2
  • Glossitis or oral symptoms 2
  • Depression, brain fog, ataxia 3

Cardiovascular implications:

  • B12 deficiency increases stroke risk through elevated homocysteine 1
  • B vitamins including B12 reduced ischemic stroke by 43% in meta-analyses 1
  • Among stroke patients, 17.3% had biochemical or metabolic B12 deficiency 1

Common Pitfalls to Avoid

Do not rely solely on serum B12 to rule out deficiency, especially if you are over 60 years old, where metabolic deficiency is common despite "normal" serum levels 2

Check for underlying causes:

  • Medications: metformin (>4 months use), proton pump inhibitors (>12 months), H2 blockers, colchicine 2, 5, 3
  • Autoimmune conditions: test for intrinsic factor antibodies and gastric parietal cell antibodies 3
  • Helicobacter pylori testing for atrophic gastritis 3
  • Dietary factors: vegetarian/vegan diet 2, 5
  • History of gastrointestinal surgery 5, 3

Special Considerations

If you are over 60 years old: You have significantly higher rates of metabolic deficiency (18.1% in those >80 years) despite "normal" serum B12 2, 4

If you have cardiovascular disease or stroke history: Treatment is particularly important as B12 deficiency increases stroke risk, and supplementation can reduce this risk 1

Duration of treatment: Continue until levels normalize, then lifelong maintenance therapy if intrinsic factor antibodies are positive or if you have pernicious anemia 2, 7

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Vitamin B12 and Magnesium Deficiency Diagnosis

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Vitamin B12 Deficiency: Common Questions and Answers.

American family physician, 2025

Guideline

Vitamin B12 Requirements and Deficiency in the Elderly

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Vitamin B12 Deficiency: Recognition and Management.

American family physician, 2017

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.