What is the most likely diagnosis of painful penile ulcers with no inguinal lymphadenopathy in a patient with a history of unprotected sex?

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Most Likely Diagnosis: Genital Herpes Simplex Virus (HSV)

In a patient presenting with painful penile ulcers without inguinal lymphadenopathy and a history of unprotected sex, genital herpes simplex virus infection is the most likely diagnosis, as it is the most common cause of genital ulcers in the United States and typically presents with painful ulcers without prominent lymphadenopathy. 1, 2

Clinical Reasoning

Why HSV is Most Likely

  • Epidemiologic predominance: HSV is the most common cause of genital ulcers in the United States, making it the statistical frontrunner in any undifferentiated case 1, 2
  • Pain profile: The presence of painful ulcers strongly favors HSV over syphilis (which typically causes painless chancres) 3
  • Absence of lymphadenopathy: This clinical feature argues against chancroid, where tender inguinal adenopathy occurs in one-third of patients and is highly suggestive of the diagnosis 3

Why Not Chancroid

While chancroid classically presents with painful ulcers, the absence of inguinal lymphadenopathy makes this diagnosis less likely 3. The CDC guidelines specifically note that the combination of painful ulcer with tender inguinal adenopathy is suggestive of chancroid, and when accompanied by suppurative adenopathy is almost pathognomonic 3. Without lymphadenopathy, chancroid drops lower on the differential.

Why Not Syphilis

Primary syphilis typically presents with a painless chancre, not painful ulcers, making it inconsistent with this presentation 3, 1

Essential Diagnostic Workup

Despite the clinical suspicion, laboratory confirmation should always be sought because clinical diagnosis alone is often inaccurate 4, 1:

  • HSV testing: PCR or culture from the ulcer base or vesicular fluid (gold standard) 4, 5
  • Syphilis serology: Mandatory to exclude co-infection, as up to 10% of genital ulcer patients have HSV co-infected with T. pallidum 3, 4
  • Darkfield microscopy or direct fluorescent antibody test for T. pallidum 3, 1
  • HIV testing: Strongly recommended, as genital ulcers are associated with increased HIV risk 3

Critical Pitfall to Avoid

Even after complete diagnostic testing, 25% of patients with genital ulcers have no laboratory-confirmed diagnosis 3, 4. Therefore, empiric treatment based on the most likely diagnosis is often necessary before test results return 3.

Treatment Approach

For Presumed HSV (First Episode)

  • Acyclovir for 7-10 days, or alternative agents famciclovir or valacyclovir 1
  • Treatment should be initiated within 72 hours of symptom onset for optimal efficacy 5

If Diagnosis Remains Unclear

The CDC recommends that when the diagnosis is uncertain, treat for the most likely diagnosis based on clinical presentation 3. Many experts recommend empiric treatment for both chancroid and syphilis if the diagnosis is unclear and the patient resides in a community with notable chancroid morbidity 3.

Co-infection Considerations

Up to 10% of patients with chancroid may be co-infected with T. pallidum or HSV, and 3-10% of all genital ulcer patients have more than one pathogen present 3. This underscores the importance of comprehensive testing rather than relying solely on clinical impression.

References

Research

Diagnosis and management of genital ulcers.

American family physician, 2012

Research

Genital Ulcers: Differential Diagnosis and Management.

American family physician, 2020

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Genital HSV-1 Infection

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Unusual Case Presentation of Genital Herpes.

Acta dermatovenerologica Croatica : ADC, 2022

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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