What is the recommended time window for thrombolysis (tissue plasminogen activator (tPA)) in acute ischemic stroke?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: December 23, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Time Window for Thrombolysis in Acute Ischemic Stroke

Intravenous tPA should be administered within 3 hours of stroke symptom onset as the primary treatment window, with an extended window of 3-4.5 hours available for carefully selected patients who meet additional exclusion criteria. 1, 2

Standard Treatment Window (0-3 Hours)

  • The 0-3 hour window represents the strongest evidence for benefit, with high-quality data showing that tPA increases the likelihood of good functional outcome (154 more excellent outcomes per 1,000 patients treated) with no increase in overall mortality. 1, 2

  • The dose is 0.9 mg/kg (maximum 90 mg), with 10% given as initial bolus and remainder infused over 1 hour. 1

  • Symptomatic intracranial hemorrhage occurs in approximately 7.9% of tPA-treated patients versus 3.5% in placebo patients within this window. 1

  • Earlier treatment within the 3-hour window produces superior outcomes - delays should be minimized with a target door-to-needle time of 30 minutes (median), with 90th percentile at 60 minutes. 1, 2

Extended Treatment Window (3-4.5 Hours)

  • The 3-4.5 hour window is supported by high-quality evidence (Class I Recommendation, Level B) but shows smaller treatment effect than the 0-3 hour window (69 more favorable events per 1,000 patients versus 154 per 1,000 in the earlier window). 1, 2

  • The ECASS-3 trial demonstrated that 52.4% of tPA-treated patients achieved excellent outcome (mRS 0-1) versus 45.2% with placebo in this extended window. 1, 2

Additional Exclusion Criteria for 3-4.5 Hour Window

The following patients must be excluded from treatment in the 3-4.5 hour window (but may be eligible in the 0-3 hour window): 1, 2

  • Age >80 years
  • Patients taking oral anticoagulants
  • Baseline NIHSS score >25
  • Combination of previous stroke AND diabetes mellitus

Beyond 4.5 Hours

  • IV tPA should NOT be administered beyond 4.5 hours based on lack of proven benefit and potential harm. 3

  • For patients presenting 4.5-6 hours after onset, treatment should focus on aspirin 160-325 mg within 48 hours and supportive care including VTE prophylaxis. 3

  • Intra-arterial thrombolysis may be considered for proximal cerebral artery occlusions within 6 hours in select cases, though this represents a different treatment modality. 1, 3

Critical Safety Considerations

  • Symptomatic ICH risk increases with delayed treatment: 2.4% (ECASS-3 definition) to 7.9% (NINDS definition) in the 3-4.5 hour window for tPA-treated patients. 1

  • Fatal ICH occurs significantly more often with thrombolytic therapy across all time-to-treatment strata up to 6 hours (OR 3.70,95% CI 2.36-5.79), with absolute risks of 3.5% with tPA versus 0.8% with placebo. 1

  • Real-world implementation data from SITS-MOST showed symptomatic ICH rates of 7.3% in routine clinical practice, comparable to randomized trial results. 1

Key Clinical Pitfalls to Avoid

  • Do not delay treatment to wait for the extended window - approximately 45% of patients treated in the 3-4.5 hour window in registry data actually arrived within 2 hours of symptom onset and could have been treated earlier. 4

  • Do not use tPA beyond 4.5 hours in routine practice - the evidence shows harm without benefit in this timeframe. 3

  • Do not forget the additional exclusion criteria when treating in the 3-4.5 hour window - protocol violations in ECASS-I resulted in 42% mortality in those who received tPA inappropriately. 1

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Window Period in Stroke: Definition and Clinical Significance

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Treatment of Ischemic Stroke Outside the 4-Hour Window

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.