What are the indications for ordering fecal (stool) examinations?

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Last updated: April 18, 2025View editorial policy

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From the Guidelines

Stool studies should be ordered for patients with diarrhea accompanied by fever, bloody or mucoid stools, severe abdominal cramping or tenderness, or signs of sepsis, as well as for immunocompromised individuals, healthcare workers, food handlers, daycare attendees, or during suspected outbreaks. These tests typically include stool culture for bacterial pathogens (Salmonella, Shigella, Campylobacter, Yersinia, C. difficile, and STEC) 1. The indications for stool studies also include patients with persistent abdominal pain, especially school-aged children with right lower quadrant pain mimicking appendicitis, and people with fever at epidemiologic risk for yersiniosis 1. Additionally, stool specimens should be tested for Vibrio species in people with large volume rice water stools or exposure to salty or brackish waters, consumption of raw or undercooked shellfish, or travel to cholera-endemic regions within 3 days prior to onset of diarrhea 1.

  • The following patient populations should be considered for stool testing:
    • Immunocompromised individuals, especially those with moderate and severe primary or secondary immune deficiencies 1
    • People with acquired immune deficiency syndrome (AIDS) with persistent diarrhea, who should undergo additional testing for other organisms including Cryptosporidium, Cyclospora, Cystoisospora, microsporidia, Mycobacterium avium complex, and cytomegalovirus 1
    • Travelers returning from endemic areas with diarrhea, who should undergo specific parasite testing 1
  • The timing of specimen collection is important, with ideally three separate stool samples collected on different days for parasitic examinations, while fresh specimens are crucial for bacterial cultures 1.
  • Patients should be instructed to avoid contaminating specimens with urine or toilet water and to refrigerate samples if immediate transport to the laboratory isn't possible 1.
  • These tests help distinguish infectious from non-infectious causes of gastrointestinal symptoms, guiding appropriate treatment decisions and preventing unnecessary antibiotic use 1.

From the Research

Indications for Ordering Stool Studies

The following are indications for ordering stool studies:

  • Severe dehydration 2, 3
  • Bloody stools 2, 3, 4
  • Persistent fever 2, 3
  • Immunocompromised patients 2, 3
  • Suspected nosocomial infection or outbreak 2
  • Red flag symptoms such as weight loss, clinical and laboratory signs of anemia, and palpable mass in the abdomen 3
  • Chronic diarrhea with unknown cause 4
  • Inflammatory bowel diseases with diarrhea 5
  • Malabsorption syndromes 6, 4
  • Gastrointestinal infections 6

Stool Tests

Stool tests can include:

  • Microscopic examination 6
  • Chemical tests 6
  • Immunologic tests 6
  • Microbiologic tests 6
  • Leukocytes and lactoferrin presence in the stools 2
  • Stool culture 2, 5
  • C. difficile testing 2, 5
  • PCR 2
  • Ova and parasites' search 2
  • Endoscopy and abdominal imaging 2

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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