What does a herpes simplex virus type 1/2 IgG result with a reflex HSV‑2 inhibition test and an index of 0.98 indicate?

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HSV-1/2 IgG with Reflex to HSV-2 Inhibition: Index 0.98

Your result of 0.98 falls in the indeterminate range (0.9-1.1), meaning this test cannot definitively determine whether you have HSV-2 infection and should not be interpreted as either positive or negative. 1

What This Index Value Means

  • An index value of 0.98 sits in the "gray zone" between negative (<0.9) and low-positive (1.1-2.9), representing an indeterminate result that requires careful interpretation 1
  • This value is too close to the cutoff threshold to reliably distinguish true infection from laboratory variability or cross-reactivity 1
  • The reflex HSV-2 inhibition test was likely performed automatically because your initial screening showed borderline reactivity, but the final index of 0.98 still falls short of the positive threshold 1

Why This Result Is Problematic

  • Commercial HSV-2 IgG tests have significant limitations, with false-positive rates as high as 60% when index values are between 1.1-2.9 1, 2
  • Even at higher index values (≥3.0), specificity only reaches 78.6%, meaning more than 1 in 5 positive results could be false 1
  • Patients with HSV-1 infection are significantly more likely to have false-positive HSV-2 results due to antibody cross-reactivity, especially at low index values 1
  • HSV-1 antibody detection itself has only 70.2% sensitivity, resulting in frequent false-negative results 1

What You Should Do Next

If You Have Never Had Symptoms:

  • Do not assume you have HSV-2 infection based on this result 1
  • If you had a potential exposure within the past 12 weeks, repeat testing after the 12-week window period has passed, as antibodies may still be developing 1, 3
  • If the exposure was more than 12 weeks ago, consider repeating the test in 4-6 weeks to see if the index value changes significantly 1

If You Have Active Genital Lesions:

  • Request PCR or viral culture testing of the lesion immediately, as this provides definitive diagnosis and is far more accurate than serology 4
  • PCR has 96-98% sensitivity and 95-99% specificity for HSV detection from active lesions, making it the gold standard for diagnosis when lesions are present 5, 4
  • Do not rely on serology alone when active lesions are available for direct testing 1, 3

If You Need Definitive Clarification:

  • Request Western blot/immunoblot testing, which is the gold standard for HSV serological testing 1, 3
  • Alternatively, request confirmation with a second assay using a different glycoprotein G antigen, which improves specificity from 93.2% to 98.7% 1
  • Be aware that even with recurrent, PCR-confirmed HSV infections, commercial antibody tests can be false-negative in 12-30% of cases 6

Critical Pitfalls to Avoid

  • Do not accept a diagnosis of HSV-2 infection based on this indeterminate result, as this can lead to significant psychological distress, relationship consequences, and unnecessary treatment 1, 3
  • Do not test again immediately—allow adequate time for antibody development if recent exposure is suspected (minimum 12 weeks) 1, 3
  • Do not use HSV-1 serology results to diagnose genital herpes, as HSV-1 is commonly acquired in childhood through non-sexual contact and cannot distinguish oral from genital infection 1, 7

Understanding the Broader Context

  • Up to 70% of adults worldwide have HSV-1 antibodies from childhood exposure 7
  • The presence of HSV-1 antibodies increases the likelihood of false-positive HSV-2 results on commercial assays 1
  • Different commercial platforms have dramatically different performance characteristics, with some showing false-positive rates approaching 31% for low-positive HSV-2 results 2

References

Guideline

Optimal Serological Testing for Herpes Simplex Virus

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Significance of Positive HSV Type 2 IgG Antibody

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Interpretation of HSV 1/2 IgG Serology Results

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

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Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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