In a newborn who is Rh‑positive (O+) delivered by an Rh‑negative (O‑) mother, should anti‑D (Rho(D) immune globulin) be given to the mother, the baby, both, or neither?

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Anti-D Administration After Delivery of Rh-Positive Baby to Rh-Negative Mother

Give anti-D to the mother only (Option A).

Rationale for Maternal Administration

Anti-D immune globulin should be administered to the Rh-negative mother within 72 hours after delivery of an Rh-positive infant to prevent RhD alloimmunization and subsequent hemolytic disease of the fetus and newborn in future pregnancies. 1, 2

The mechanism works by suppressing the maternal immune response to fetal RhD-positive red blood cells that enter maternal circulation during delivery. 1 Without prophylaxis, approximately 13-17% of Rh-negative mothers delivering Rh-positive infants will develop anti-D antibodies, but postpartum RhIg reduces this risk to 1-2%. 1

Why the Baby Does Not Receive Anti-D

The newborn should never receive anti-D immune globulin. 2 The FDA-approved indication specifically states that anti-D is administered to the Rho(D)-negative mother, not the infant. 2 The baby's Rh-positive red blood cells are the target antigen that could trigger maternal sensitization—giving anti-D to the baby serves no protective purpose and is not indicated. 2

Critical Eligibility Criteria

Before administering anti-D to the mother, verify: 2

  • The mother must be Rho(D)-negative
  • The mother must not already be sensitized (no pre-existing anti-D antibodies)
  • The infant must be Rho(D)-positive
  • The infant should have a negative direct antiglobulin test (DAT)

Timing Considerations

The 72-hour window is critical for optimal effectiveness. 1, 2 However, if this window is missed, anti-D should still be given as soon as recognized, up to 28 days after delivery, as delayed administration provides some benefit even though protection decreases over time. 1

Dosing for Standard Delivery

The standard dose is 300 μg (1500 IU) administered intramuscularly or intravenously. 1, 2 Both routes are equally effective based on available evidence. 3

When Additional Doses Are Required

If fetomaternal hemorrhage exceeds 15 mL of fetal red blood cells (approximately 30 mL of fetal blood), additional anti-D is required at 10 μg per 0.5 mL of fetal red blood cells. 1, 4 Events with substantial placental trauma—such as placental abruption, abdominal trauma, or complicated deliveries—warrant consideration of quantitative testing for fetomaternal hemorrhage to determine if supplemental dosing is needed. 1, 4

Common Pitfall to Avoid

Do not withhold anti-D if the father's Rh status is unknown or if paternity is uncertain—always assume the infant is Rh-positive unless proven otherwise. 2, 4 The consequences of sensitization (severe HDFN in subsequent pregnancies, need for fetal transfusions, hydrops, stillbirth) far outweigh the minimal risks of anti-D administration. 1

References

Guideline

Management Guidelines for Rh Negative Pregnancy

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Prevention of Rh alloimmunization.

Journal of obstetrics and gynaecology Canada : JOGC = Journal d'obstetrique et gynecologie du Canada : JOGC, 2003

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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