What is non‑alcoholic steatohepatitis (NASH)?

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What is NASH (Nonalcoholic Steatohepatitis)?

NASH is a progressive form of fatty liver disease defined by the presence of ≥5% hepatic steatosis combined with inflammation and hepatocyte injury (ballooning), with or without fibrosis, occurring in individuals without significant alcohol consumption. 1

Histologic Definition and Diagnostic Criteria

NASH requires liver biopsy for definitive diagnosis and is characterized by three key histologic features 1:

  • Macrovesicular steatosis (≥5% of hepatocytes containing fat)
  • Hepatocyte ballooning (cellular injury with swelling)
  • Lobular inflammation (mixed inflammatory infiltrate including neutrophils)
  • Zone 3 perisinusoidal fibrosis may be present but is not required for diagnosis 1

Additional histologic features frequently seen but not required include Mallory-Denk bodies (damaged intermediate filament inclusions) and acidophil bodies (apoptotic hepatocytes) 1.

Distinction from Simple Fatty Liver

NASH differs critically from nonalcoholic fatty liver (NAFL) in both histology and prognosis 1:

  • NAFL: ≥5% steatosis without hepatocyte ballooning or significant inflammation; minimal progression risk
  • NASH: ≥5% steatosis WITH inflammation and ballooning; can progress to cirrhosis, liver failure, and hepatocellular carcinoma 1

The presence of hepatocyte ballooning is the key distinguishing feature that separates NASH from NAFL 1.

Clinical Significance and Natural History

NASH carries substantial morbidity and mortality risk 2:

  • Affects 3-6% of the US population (approximately 20% of patients with NAFLD have NASH) 1, 2
  • 20% of NASH patients will develop cirrhosis 2
  • All-cause mortality rate: 25.56 per 1,000 person-years 2
  • Liver-specific mortality rate: 11.77 per 1,000 person-years 2
  • NASH is the third leading cause for liver transplant in the United States and predicted to become the leading indication 1, 2

Fibrosis Progression

Approximately 30-40% of NASH patients develop progressive fibrosis 1:

  • Fibrosis progression rate from stage 0 to stage 1: 7 years for NASH versus 14 years for NAFL 1
  • Advanced fibrosis (bridging fibrosis or cirrhosis) significantly increases risk of hepatic decompensation and hepatocellular carcinoma 1
  • 2-3% of patients with NASH cirrhosis develop hepatocellular carcinoma annually 1

Associated Metabolic Conditions

NASH is strongly associated with metabolic dysfunction 1, 2:

  • Obesity (most common association)
  • Type 2 diabetes mellitus
  • Dyslipidemia
  • Metabolic syndrome
  • Insulin resistance

These metabolic comorbidities are not merely associations but active drivers of disease progression 1.

Pathophysiologic Mechanisms

NASH develops through multiple parallel mechanisms 3, 4:

  • Lipotoxicity: Accumulation of toxic lipid species beyond simple triglycerides
  • Oxidative stress and ER stress: Drive hepatocellular injury 3, 5
  • Hepatocyte apoptosis: Key contributor to liver injury 3
  • Kupffer cell activation: Initiates and perpetuates inflammatory response 3
  • Extrahepatic factors: Adipose tissue dysfunction, gut microbiome alterations, and gastrointestinal hormones contribute to disease progression 3, 4

Important Clinical Caveats

Alcohol consumption thresholds must be excluded for NASH diagnosis: <30 g/day for men and <20 g/day for women 6. Secondary causes of hepatic steatosis (steatogenic medications, hereditary disorders) must also be ruled out 1.

NASH can progress even from bland steatosis, particularly in the presence of metabolic risk factors, challenging the traditional view of NAFL and NASH as separate entities rather than a disease continuum 1.

Hepatocellular carcinoma can develop in NASH patients without cirrhosis, though the risk appears low and routine surveillance in non-cirrhotic NASH is not currently recommended 1.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Pathophysiology of NASH: perspectives for a targeted treatment.

Current pharmaceutical design, 2013

Research

Triggering and resolution of inflammation in NASH.

Nature reviews. Gastroenterology & hepatology, 2018

Guideline

Diagnostic Approach and Management of Metabolic Fatty Liver Disease

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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