How many International Units (IU) are equivalent to 0.25 micrograms (µg) of vitamin D₃?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: February 15, 2026View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Vitamin D3 Conversion: Micrograms to International Units

0.25 micrograms (mcg) of vitamin D3 equals 10 International Units (IU).

Standard Conversion Formula

The universally accepted conversion for vitamin D3 (cholecalciferol) is straightforward 1:

  • 40 IU = 1 mcg 1
  • Therefore: 0.25 mcg × 40 = 10 IU

This conversion factor is consistent across all clinical guidelines and applies to both vitamin D3 (cholecalciferol) and vitamin D2 (ergocalciferol) when measuring potency 1.

Clinical Context

To put this dose in perspective:

  • Standard infant supplementation is 400 IU/day (10 mcg/day), which equals 40 times this amount 2
  • Standard adult maintenance ranges from 600-800 IU/day (15-20 mcg/day), which equals 60-80 times this amount 1
  • Therapeutic loading doses for deficiency are typically 50,000 IU weekly (1,250 mcg weekly), which equals 5,000 times this amount 1, 3

A dose of 0.25 mcg (10 IU) represents a negligible amount of vitamin D3 and would have no meaningful clinical effect on vitamin D status 1, 3.

Important Conversion Notes

  • This conversion applies specifically to cholecalciferol (vitamin D3), which is the preferred form for supplementation due to its superior potency and longer duration of action compared to ergocalciferol (vitamin D2) 4, 5
  • When reading supplement labels or prescriptions, always verify whether the dose is expressed in micrograms (mcg) or International Units (IU), as confusion between these units can lead to significant dosing errors 1
  • The conversion remains constant regardless of the route of administration (oral, intramuscular, or intravenous) 1, 3

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Vitamin D Supplementation in Infants

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

Vitamin D Insufficiency Treatment Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Research

Vitamin D(3) is more potent than vitamin D(2) in humans.

The Journal of clinical endocrinology and metabolism, 2011

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.