How is Human Papillomavirus (HPV) detected in males?

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From the Guidelines

There is no routine screening test for HPV in men, and diagnosis typically occurs when visible symptoms appear, such as genital warts, which can be identified through a visual examination of the genital area or a biopsy 1. Unlike women who can be tested through Pap smears, men do not have a standard screening method for HPV. Healthcare providers may perform a visual examination of the genital area to check for warts or abnormal tissue. In some cases, a doctor might apply a vinegar solution (acetic acid) to help identify flat warts that aren't easily visible. For men who have sex with men or those who are immunocompromised, some doctors recommend anal Pap tests, though these aren't standard practice. If warts are present, a biopsy might be taken to confirm HPV infection. Blood tests don't detect HPV, and there's no FDA-approved test to detect the virus in men without symptoms. This lack of testing options exists because HPV infections in men often clear on their own without causing health problems, though they can still transmit the virus to partners. Some key points to consider when checking for HPV in men include:

  • The importance of visual examination and biopsy in diagnosing HPV-related genital warts 1
  • The lack of standard screening methods for HPV in men, unlike women who can undergo Pap smears 1
  • The potential benefits of anal Pap tests for men who have sex with men or are immunocompromised, although this is not a standard practice 1
  • The fact that HPV infections in men often clear on their own without causing health problems, but can still be transmitted to partners 1. The best prevention remains vaccination before sexual activity begins, as the HPV vaccine protects against the most high-risk strains of the virus 1.

From the Research

Checking for HPV in Men

To check for HPV in men, several methods can be used, including:

  • Sampling from the penile shaft, glans, prepuce, coronal sulcus, urine, and semen 2
  • Reverse line blot (RLB) and hybrid capture (HC) techniques for detection 2
  • Anal Pap test screening, which involves the blind insertion of a swab into the anal canal and fixing cells for cytological examination 3
  • High-resolution anoscopy (HRA)-guided biopsy, which is considered the diagnostic standard 3
  • HPV DNA testing, which can be used to detect and differentiate HPV viral strains 3

Risk Factors and Screening

Certain groups of men are at higher risk for anal cancer and HPV infection, including:

  • HIV-positive men, particularly those who have sex with men 3, 4
  • Men with immune dysfunctions 3
  • Men with a history of genital warts or other sexually transmitted infections 4, 5 Screening for anal cancer and HPV infection is important for these high-risk groups, and can include:
  • Annual anal Pap testing 3
  • HPV DNA testing 3, 6
  • HRA-guided biopsy 3, 6

Diagnostic Performance

The diagnostic performance of different screening methods varies, with:

  • Anal cytology having low sensitivity (59%) but high specificity (73%) 6
  • HR-HPV testing having higher sensitivity (85%) but lower specificity (43%) when used alone 6
  • Cotesting (combining anal cytology and HR-HPV testing) having the highest sensitivity (85%) but lowest specificity (43%) 6
  • HR-HPV testing being useful for triaging patients with normal and atypical squamous cells of undetermined significance cytology for HRA 6

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

[Detection of human papilloma virus infection in men].

Revista chilena de infectologia : organo oficial de la Sociedad Chilena de Infectologia, 2013

Research

Anal dysplasia screening: an evidence-based analysis.

Ontario health technology assessment series, 2007

Research

Perianal warts as a risk marker for anal high-risk-human papillomavirus (HPV) detection and HPV-associated diseases.

Journal of the European Academy of Dermatology and Venereology : JEADV, 2020

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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