What is the dosage for HIV post-exposure prophylaxis (PEP)?

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Last updated: October 16, 2025View editorial policy

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HIV Post-Exposure Prophylaxis (PEP) Dosage Recommendations

The preferred HIV post-exposure prophylaxis (PEP) regimens for most adults and adolescents are bictegravir/emtricitabine/tenofovir alafenamide (BIC/FTC/TAF) as a single-tablet regimen, or dolutegravir (DTG) plus (tenofovir alafenamide [TAF] or tenofovir disoproxil fumarate [TDF]) plus (emtricitabine [FTC] or lamivudine [3TC]), administered for a 28-day course. 1, 2

Timing and Duration

  • PEP should be initiated as soon as possible after exposure, ideally within 24 hours, but no later than 72 hours 1, 2
  • The first dose should not be delayed due to pending laboratory test results 1
  • The complete course of PEP is 28 days 1, 2

Specific Regimen Details

Preferred Regimens for Adults and Adolescents:

  • Option 1: Bictegravir/emtricitabine/tenofovir alafenamide (BIC/FTC/TAF) - single tablet regimen 1, 2
  • Option 2: Dolutegravir (DTG) plus (tenofovir alafenamide [TAF] OR tenofovir disoproxil fumarate [TDF]) plus (emtricitabine [FTC] OR lamivudine [3TC]) 1, 2

Alternative Regimens (when preferred regimens are not available):

  • Older guidelines suggested zidovudine (ZDV) + lamivudine (3TC) as the backbone with lopinavir/ritonavir (LPV/r) or atazanavir/ritonavir (ATV/r) as the third drug, but these are now considered outdated 1, 3

Follow-up and Monitoring

  • Clinical follow-up should occur at 24 hours (remote or in-person) with a medical provider 1
  • Laboratory testing should be performed at 4-6 weeks and 12 weeks after exposure 1, 2
  • HIV testing should include laboratory-based HIV Ag/Ab combination immunoassay and diagnostic HIV NAT at 12 weeks post-exposure 2
  • For persons with long-acting injectable PrEP exposure in the past 12 months, add a diagnostic HIV nucleic acid test (NAT) at initial assessment 2

Special Considerations

  • For pregnant individuals, the regimen should be tailored to clinical circumstances, though older guidelines considered ZDV + 3TC a safe regimen for pregnant healthcare workers 3
  • For individuals with renal impairment, TAF-containing regimens may be preferred over TDF-containing regimens 3, 2
  • Assess for medical comorbidities, current medications, and potential drug interactions at the initial assessment 2

Transition to PrEP

  • Consider immediate transition from PEP to PrEP for persons with anticipated repeat or ongoing potential HIV exposures 2
  • Perform HIV testing at the completion of the PEP regimen before transitioning to PrEP 2

Common Pitfalls to Avoid

  • Delaying PEP initiation beyond 72 hours significantly reduces effectiveness 2, 4
  • Incomplete adherence to the full 28-day course reduces effectiveness 2, 4
  • Failing to consider drug interactions with the patient's current medications 3, 2
  • Not providing adequate follow-up for medication adherence and toxicity monitoring 2

It's important to note that the recommendations have evolved significantly over time, with a shift from older zidovudine-based regimens to newer tenofovir-based regimens with integrase inhibitors, which offer better tolerability and completion rates 4.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Post-Exposure Prophylaxis for HIV Exposure

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Post-Exposure Prophylaxis Regimens and Management

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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