What is the significance of an elevated Centromere Protein B (CENP-B) antibody level?

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Last updated: October 22, 2025View editorial policy

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Significance of Elevated Centromere Protein B Antibody

Elevated anti-centromere protein B (CENP-B) antibody is most strongly associated with limited systemic sclerosis (formerly CREST syndrome) and indicates a high risk for developing Raynaud's phenomenon and other features of limited scleroderma. 1, 2

Clinical Significance and Disease Associations

  • Anti-CENP-B antibodies are a specific type of anti-nuclear antibody (ANA) that target centromere protein B, which is an alphoid DNA binding protein 3
  • These antibodies are primarily associated with limited cutaneous systemic sclerosis (lcSSc), formerly known as CREST syndrome (calcinosis, Raynaud's phenomenon, esophageal dysfunction, sclerodactyly, and telangiectasia) 2, 4
  • The presence of anti-CENP-B antibodies is significantly more common in limited systemic sclerosis (48%) compared to diffuse systemic sclerosis (9-11%) 5
  • Anti-CENP-B antibodies have high disease specificity (95-96.5%) for systemic sclerosis when compared to other autoimmune conditions 6, 5

Diagnostic Value

  • Anti-CENP-B antibody testing has approximately 98% sensitivity and 95% specificity for detecting anticentromere antibodies in patients at risk for systemic sclerosis 6
  • These antibodies serve as a useful prognostic indicator in early scleroderma, helping to predict the pattern of disease that will develop 2
  • Anti-CENP-B antibodies are rarely found in patients with rapidly advancing or diffuse scleroderma, making their presence a marker for a more limited disease course 2
  • The antibody levels correlate with the presence and severity of Raynaud's phenomenon, suggesting a potential pathogenic role 3

Clinical Overlap Syndromes

  • Anti-CENP-B antibodies can identify patients with Sjögren's syndrome who have autoimmune overlap syndromes 4
  • Patients with Sjögren's syndrome who are positive for anti-CENP-B show greater frequency of:
    • Raynaud's phenomenon
    • Objective xerophthalmia (dry eyes)
    • Peripheral neuropathy
    • Additional autoimmune disorders, especially primary biliary cirrhosis 4
  • These patients often have fewer traditional Sjögren's antibodies (anti-SSA/SSB) but a greater prevalence of other autoantibodies 4

Laboratory Testing Considerations

  • Anti-CENP-B antibodies are typically detected as part of an ANA panel with a characteristic centromere pattern on immunofluorescence 1
  • ELISA tests for CENP-B yield results with similar sensitivity and specificity as immunofluorescence for the diagnosis of systemic sclerosis 5
  • The optimal screening dilution for ANA testing should be defined locally, with 1:160 often being the most suitable for adult patients 1
  • In patients with systemic sclerosis, the autoantibody response is typically directed simultaneously against both CENP-A and CENP-B components of the centromere structure 5

Clinical Management Implications

  • Screening for anti-CENP-B antibodies should be conducted in all patients with Raynaud's phenomenon, primary biliary cirrhosis, and scleroderma 2
  • The presence of these antibodies in a patient with early symptoms should prompt close monitoring for the development of limited systemic sclerosis 2, 4
  • Patients with positive anti-CENP-B antibodies should be evaluated for associated conditions, particularly primary biliary cirrhosis and other autoimmune disorders 4
  • Regular assessment for progression of symptoms related to limited systemic sclerosis is warranted in antibody-positive individuals 3, 2

Understanding the significance of elevated anti-CENP-B antibodies allows for early identification of patients at risk for limited systemic sclerosis and associated overlap syndromes, enabling appropriate monitoring and management strategies.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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